Korean J Nutr.  2011 Oct;44(5):378-383. 10.4163/kjn.2011.44.5.378.

The Effect of Supplementary Selenium on Leukocytes and HSP70 Expression after Half-Body Immersion

• Shin YO
• Han MK
• Lee JB
• Um BH

• KMID: 2268561
• DOI: http://doi.org/10.4163/kjn.2011.44.5.378

Abstract

This study examined the effect of supplementary selenium on leukocytes and heat shock protein (HSP) 70 expression in serum during half-body immersion. The subjects were male college tennis athletes. All subjects participated in two repeated experiments with a 1 week interval. During the 30 min intermittent half-body immersion, subjects were given 500 mL of water with or without selenium (100 microg). Blood samples were taken from the antecubital vein, and differential counts were made. Serum HSP70 protein was analyzed using a commercial ELISA kit. After half-body immersion, leukocytes and lymphocytes increased significantly but neutrophils decreased significantly in both trials (with or without selenium). Selenium supplementation, compared with placebo, decreased levels of leukocytes, neutrophils, and monocytes, but not lymphocytes, to the resting level or below 60 min after immersion. Only lymphocytes continued to increase in both trials during the recovery period. Serum HSP70 protein level did not change after immersion, but it decreased 60 min after immersion with the administration of selenium. In conclusion, supplementary selenium reduced the systemic immune response and serum HSP70 protein accumulation after half-body immersion.

Keyword

half-body immersion; selenium; serum; HSP70; leukocytes

MeSH Terms

Athletes
Enzyme-Linked Immunosorbent Assay
Heat-Shock Proteins
Humans
Immersion
Leukocytes
Lymphocytes
Male
Monocytes
Neutrophils
Selenium
Tennis
Veins
Water
Heat-Shock Proteins
Selenium
Water

Figure

• 

  Fig. 1 Changes of leukocytes and subsets over time in two treatments. White bar: water and placebo, black bar: water and selenium. *: p < 0.05, **: p <0.01, ***: p <0.001; compare with Pre in each treatment.

• 

  Fig. 2 HSP70 expression over time in two treatments. White bar: water and placebo, black bar: water and selenium. *: p <0.05. compare with Pre in each treatment.

Reference

1. Rayman MP. The importance of selenium to human health. Lancet. 2000. 356(9225):233–241.

2. Turner RJ, Finch JM. Selenium and the immune response. Proc Nutr Soc. 1991. 50(2):275–285.

3. Kiremidjian-Schumacher L, Roy M. Selenium and immune function. Z Ernahrungswiss. 1998. 37:Suppl 1. 50–56.

4. McKenzie RC, Arthur JR, Miller SM, Rafferty TS, Beckett GJ. Calder PC, Field CJ, Gil NS, editors. Selenium and the immune system. Nutrition and Immune Function. 2002. Oxford, U.K.: CAB International;229–250.

5. Beckett GJ, Arthur JR, Miller SM, McKenzie RC. Hughes DA, Bendich A, Darlington G, editors. Selenium, immunity and disease. Dietary Enhancement of Human Immune Function. 2003. Totowa, NJ: Humana Press.

6. Bouchama A, al Hussein K, Adra C, Rezeig M, al Shail E, al Sedairy S. Distribution of peripheral blood leukocytes in acute heatstroke. J Appl Physiol. 1992. 73(2):405–409.

7. Kappel M, Diamant M, Hansen MB, Klokker M, Pedersen BK. Effects of in vitro hyperthermia on the proliferative response of blood mononuclear cell subsets, and detection of interleukins 1 and 6, tumour necrosis factor-alpha and interferon-gamma. Immunology. 1991. 73(3):304–308.

8. Kappel M, Kharazmi A, Nielsen H, Gyhrs A, Pedersen BK. Modulation of the counts and functions of neutrophils and monocytes under in vivo hyperthermia conditions. Int J Hyperthermia. 1994. 10(2):165–173.

9. Pedersen BK, Kappel M, Klokker M, Nielsen HB, Secher NH. The immune system during exposure to extreme physiologic conditions. Int J Sports Med. 1994. 15:Suppl 3. S116–S121.

10. Oehler R, Pusch E, Zellner M, Dungel P, Hergovics N, Homoncik M, Eliasen MM, Brabec M, Roth E. Cell type-specific variations in the induction of hsp70 in human leukocytes by feverlike whole body hyperthermia. Cell Stress Chaperones. 2001. 6(4):306–315.

11. Lovell R, Madden L, McNaughton LR, Carroll S. Effects of active and passive hyperthermia on heat shock protein 70 (HSP70). Amino Acids. 2008. 34(2):203–211.

12. Rivera RE, Christensen VL, Edens FW, Wineland MJ. Influence of selenium on heat shock protein 70 expression in heat stressed turkey embryos (Meleagris gallopavo). Comp Biochem Physiol A Mol Integr Physiol. 2005. 142(4):427–432.

13. Mahmoud KZ, Edens FW. Influence of organic selenium on hsp70 response of heat-stressed and enteropathogenic Escherichia coli-challenged broiler chickens (Gallus gallus). Comp Biochem Physiol C Toxicol Pharmacol. 2005. 141(1):69–75.

14. The Korean Nutrition Society. Dietary reference intakes for Koreans. 2010.

15. Gapen CJ, Moseley PL. Acidosis alters the hyperthermic cytotoxicity and the cellular stress response. J Therm Biol. 1995. 20(4):321–325.

16. Kiang JG, Carr FE, Burns MR, McClain DE. HSP-72 synthesis is promoted by increase in [Ca2+]i or activation of G proteins but not pHi or cAMP. Am J Physiol. 1994. 267(1 Pt 1):C104–C111.

17. Febbraio MA, Mesa JL, Chung J, Steensberg A, Keller C, Nielsen HB, Krustrup P, Ott P, Secher NH, Pedersen BK. Glucose ingestion attenuates the exercise-induced increase in circulating heat shock protein 72 and heat shock protein 60 in humans. Cell Stress Chaperones. 2004. 9(4):390–396.

18. Benjamin IJ, Kröger B, Williams RS. Activation of the heat shock transcription factor by hypoxia in mammalian cells. Proc Natl Acad Sci U S A. 1990. 87(16):6263–6267.

19. Fehrenbach E, Niess AM, Schlotz E, Passek F, Dickhuth HH, Northoff H. Transcriptional and translational regulation of heat shock proteins in leukocytes of endurance runners. J Appl Physiol. 2000. 89(2):704–710.

20. Fehrenbach E, Passek F, Niess AM, Pohla H, Weinstock C, Dickhuth HH, Northoff H. HSP expression in human leukocytes is modulated by endurance exercise. Med Sci Sports Exerc. 2000. 32(3):592–600.

21. Shin YO, Oh JK, Bae JS, Lee YM, Lee JB, Yang HM, Min YK, Matsumoto T. Expression of exercise-induced HSP70 in long-distance runner's leukocytes. J Therm Biol. 2004. 29(7-8):769–774.

22. Kelly KW. Moberg GP, editor. Immunological consequences of changing environmental stimuli. Animal Stress. 1985. Bethesda, Maryland: American Physiological Society;193–224.

23. Jacobsen KL. The well-being of dairy cows in hot and humid climates. Part I. Housing and the effects of heat stress. Compend Contin Educ Pract Vet. 1996. 18(4):S137–S143.

24. Shrieve DC, Li GC, Astromoff A, Harris JW. Cellular glutathione, thermal sensitivity, and thermotolerance in Chinese hamster fibroblasts and their heat-resistant variants. Cancer Res. 1986. 46(4 Pt 1):1684–1687.

25. Skibba JL, Quebbeman EJ, Kalbfleisch JH. Nitrogen metabolism and lipid peroxidation during hyperthermic perfusion of human livers with cancer. Cancer Res. 1986. 46(11):6000–6003.

26. Ando M, Katagiri K, Yamamoto S, Asanuma S, Usuda M, Kawahara I, Wakamatsu K. Effect of hyperthermia on glutathione peroxidase and lipid peroxidative damage in liver. J Therm Biol. 1994. 19(3):177–185.