Ann Dermatol.  2015 Apr;27(2):230-232. 10.5021/ad.2015.27.2.230.

Influence of Pulse Type on Subcellular Selective Photothermolysis of Melanosomes in Adult Zebrafish Skin Following 1,064-nm, Q-switched, Nd:YAG Laser Irradiation: A Pilot Study

• Yoo MG
• So BJ
• Lee JM
• Kim DH
• Park HC
• Ryu HJ
• Kim IH

• KMID: 2264824
• DOI: http://doi.org/10.5021/ad.2015.27.2.230

Abstract

No abstract available.

MeSH Terms

Adult*
Humans
Melanosomes*
Pilot Projects*
Skin*
Zebrafish*

Figure

• 

  Fig. 1 Changes over time in melanosomes at the site of laser irradiation (×12.5). For the conventional and dual-pulse type lasers, a fluence of 0.5 J/cm2 was sufficient for the near complete elimination of adult zebrafish melanosomes. Melanosomes regenerated over time.

• 

  Fig. 2 Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and 4',6-diamidino-2-phenylindole (DAPI) staining of zebrafish skin tissue 1 day after laser irradiation (×20). DAPI staining (blue) shows normal and apoptotic nuclei. TUNEL staining (red) shows apoptotic nuclei. No apoptotic cells were detected with either pulse type at a fluence of 0.4 J/cm2. Apoptotic cells were present in the conventional pulse type, and no apoptosis was observed with the dual-pulse type at a fluence of 0.5 J/cm2.

Reference

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