Korean J Anesthesiol.  1994 Nov;27(11):1541-1550. 10.4097/kjae.1994.27.11.1541.

The Effect of Tetraethylammonium Chloride on the Impulse Conduction Block by bupivacaine

• Lee SJ
• Kim BJ
• Choi H
• Lee SW
• Kil HY
• Yoon YJ

• KMID: 2236922
• DOI: http://doi.org/10.4097/kjae.1994.27.11.1541

Abstract

Potassium channel blockers slow depolarization, broaden the action potential, and thus pro- mote the open and inactivated Na+ channel states. The ability of local anesthetics to reduce the amplitude of compound action potential(CAP) of rat sciatic nerve was examined in the presence and absence of teteraethylammonium chloride(TEA) that selectively block K+ channels, In the presence of 1.3X10-5 M bupivacaine that inhibit the CAP by 22.5% at tonic stimulation, the addition of TEA(10-1M) increased this inhibition by another 27.5% and increased another 50% by phasic stimulation(20Hz). Also, dose response curve of bupivacaine in the presence of TEA(10-1M) showed marked shift to left of curve. The re- covery kinetics of bupivacaine in the presence of various coneentration of TEA(10-2-10-1M) showed marked delay of recovery(2X10-2 M), reocurrence of inhibition(90min,5X10-2 M), even no recovery(10-1M). TEA alone slightly depolarized the resting membrane which was represented as increment of CAP height from 0.9%(3min) to 12.3%(80min), and broadened mid-peak amplitude width by 2 times in 5X10-1M, 5.3 times in 1M. These experiments directly demonstrated that TEA potentiated the inhibition of CAP by bupivacaine and showed the poesibility of mixture of TEA and local anesthetics to potenti- ate impulse conduction blockade.

Keyword

K+channel blocker; Tetraethylammonium chloride; Bupivacaine; Compound action potential

MeSH Terms

Action Potentials
Anesthetics, Local
Animals
Bupivacaine*
Kinetics
Membranes
Potassium Channel Blockers
Rats
Sciatic Nerve
Tea
Tetraethylammonium*
Anesthetics, Local
Bupivacaine
Potassium Channel Blockers
Tea
Tetraethylammonium