Gut Liver.  2015 Sep;9(5):665-671. 10.5009/gnl14212.

Potential Serum Markers for Monitoring the Progression of Hepatitis B Virus-Associated Chronic Hepatic Lesions to Liver Cirrhosis

• Wu C
• Liu L
• Zhao P
• Tang D
• Yao D
• Zhu L
• Wang Z

• KMID: 2234074
• DOI: http://doi.org/10.5009/gnl14212

Abstract

BACKGROUND/AIMS
To screen for serum protein/peptide biomarkers of hepatitis B virus (HBV)-associated chronic hepatic lesions in an attempt to profile the progression of HBV-associated chronic hepatic lesions using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) techniques.
METHODS
Using SELDI-TOF MS, serum protein/peptide profiles on the CM10 ProteinChip arrays were obtained from a training group including 26 HBV-associated hepatocellular carcinoma patients with liver cirrhosis (LC), 30 HBV-associated LC patients, 85 patients at different stages of liver fibrosis, and 30 asymptomatic HBV carriers. The most valuable SELDI peak for predicting the progression to LC in HBV-infected patients was identified.
RESULTS
A SELDI peak of M/Z 5805 with value for predicting LC in HBV-infected patients was found and was identified as a peptide of the C-terminal fraction of the fibrinogen alpha-chain precursor, isoform 1.
CONCLUSIONS
The peptide of the C-terminal fraction of the fibrinogen alpha-chain precursor, isoform 1 with M/Z 5805, may be a serological biomarker for progression to LC in HBV-infected patients.

Keyword

Chronic hepatic lesions; Proteomics; Diagnosis; Surface-enhanced laser desorption/ionization; Biological markers

MeSH Terms

Adult
Aged
Biomarkers/*blood
Carcinoma, Hepatocellular/*virology
Disease Progression
Female
Hepatitis B virus
Hepatitis B, Chronic/*blood/complications
Humans
Liver Cirrhosis/*blood/pathology/virology
Liver Neoplasms/*virology
Male
Middle Aged
Protein Array Analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Young Adult
Biomarkers