J Adv Prosthodont.  2014 Apr;6(2):88-95. 10.4047/jap.2014.6.2.88.

The effect of dentin desensitizers and Nd:YAG laser pre-treatment on microtensile bond strength of self-adhesive resin cement to dentin

• Acar O
• Tuncer D
• Yuzugullu
• Celik C

• KMID: 2233335
• DOI: http://doi.org/10.4047/jap.2014.6.2.88

Abstract

PURPOSE
The purpose of this study is to evaluate if pre-treatment with desensitizers have a negative effect on microtensile bond strength before cementing a restoration using recently introduced self-adhesive resin cement to dentin.
MATERIALS AND METHODS
Thirty-five human molars' occlusal surfaces were ground to expose dentin; and were randomly grouped as (n=5); 1) Gluma-(Glutaraldehyde/HEMA) 2) Aqua-Prep F-(Fluoride), 3) Bisblock-(Oxalate), 4) Cervitec Plus-(Clorhexidine), 5) Smart protect-(Triclosan), 6) Nd:YAG laser, 7) No treatment (control). After applying the selected agent, RelyX U200 self-adhesive resin cement was used to bond composite resin blocks to dentin. All groups were subjected to thermocycling for 1000 cycles between 5-55degrees C. Each bonded specimen was sectioned to microbars (6 mm x 1 mm x 1 mm) (n=20). Specimens were submitted to microtensile bond strength test at a crosshead speed of 0.5 mm/min. Kolmogorov-Smirnov, Levene's test, Kruskal-Wallis One-way Analysis of Variance, and Conover's nonparametric statistical analysis were used (P<.05).
RESULTS
Gluma, Smart Protect and Nd:YAG laser treatments showed comparable microtensile bond strengths compared with the control group (P>.05). The microtensile bond strengths of Aqua-Prep F, and Cervitec Plus were similar to each other but significantly lower than the control group (P<.05). Bisblock showed the lowest microtensile bond strength among all groups (P<.001). Most groups showed adhesive failure.
CONCLUSION
Within the limitation of this study, it is not recommended to use Aqua-prep F, Cervitec Plus and Bisblock on dentin when used with a self-adhesive resin cement due to the decrease they cause in bond strength. Beside, pre-treatment of dentin with Gluma, Smart protect, and Nd:YAG laser do not have a negative effect.

Keyword

Desensitizer agents; Dentin pretreatment; Nd:YAG laser; Self-adhesive resin cement; Microtensile bond strength; Adhesive cementation

MeSH Terms

Adhesives
Dentin*
Humans
Resin Cements*
Adhesives
Resin Cements

Figure

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  Fig. 1 Microtensile bond strength values (MPa) of self-adhesive resin cement to pretreated dentin with desensitizers. The horizontal lines in the middle of each box indicates the median microtensile bond strength values, while the top and bottom borders of the box mark the 25th and 75th percentiles, respectively. The whiskers above and below the box mark indicates the maximum and minimum levels. The same upper cases indicate no statistically significant difference between groups (P>.05).

Reference

1. Felton DA, Bergenholtz G, Kanoy BE. Evaluation of the desensitizing effect of Gluma Dentin Bond on teeth prepared for complete-coverage restorations. Int J Prosthodont. 1991; 4:292–298.

2. Brännström M, Aström A. The hydrodynamics of the dentine; its possible relationship to dentinal pain. Int Dent J. 1972; 22:219–227.

3. Gillam DG. Current diagnosis of dentin hypersensitivity in the dental office: an overview. Clin Oral Investig. 2013; 17:S21–S29.

4. Miglani S, Aggarwal V, Ahuja B. Dentin hypersensitivity: Recent trends in management. J Conserv Dent. 2010; 13:218–224.

5. Dondi dall'Orologio G, Lorenzi R, Anselmi M, Opisso V. Dentin desensitizing effects of Gluma Alternate, Health-Dent Desensitizer and Scotchbond Multi-Purpose. Am J Dent. 1999; 12:103–106.

6. Pillon FL, Romani IG, Schmidt ER. Effect of a 3% potassium oxalate topical application on dentinal hypersensitivity after subgingival scaling and root planing. J Periodontol. 2004; 75:1461–1464.

7. Gaffar A. Treating hypersensitivity with fluoride varnish. Compend Contin Educ Dent. 1999; 20:27–33.

8. Dondi dall'Orologio G, Lone A, Finger WJ. Clinical evaluation of the role of glutardialdehyde in a one-bottle adhesive. Am J Dent. 2002; 15:330–334.

9. Orchardson R, Gillam DG. Managing dentin hypersensitivity. J Am Dent Assoc. 2006; 137:990–998.

10. Fardal O, Turnbull RS. A review of the literature on use of chlorhexidine in dentistry. J Am Dent Assoc. 1986; 112:863–869.

11. Wara-aswapati N, Krongnawakul D, Jiraviboon D, Adulyanon S, Karimbux N, Pitiphat W. The effect of a new toothpaste containing potassium nitrate and triclosan on gingival health, plaque formation and dentine hypersensitivity. J Clin Periodontol. 2005; 32:53–58.

12. Kimura Y, Wilder-Smith P, Yonaga K, Matsumoto K. Treatment of dentine hypersensitivity by lasers: a review. J Clin Periodontol. 2000; 27:715–721.

13. Gholami GA, Fekrazad R, Esmaiel-Nejad A, Kalhori KA. An evaluation of the occluding effects of Er;Cr:YSGG, Nd:YAG, CO₂ and diode lasers on dentinal tubules: a scanning electron microscope in vitro study. Photomed Laser Surg. 2011; 29:115–121.

14. Matsumoto K, Kimura Y. Laser therapy of dentin hypersensitivity. J Oral Laser Appl. 2007; 7:7–25.

15. Behr M, Rosentritt M, Regnet T, Lang R, Handel G. Marginal adaptation in dentin of a self-adhesive universal resin cement compared with well-tried systems. Dent Mater. 2004; 20:191–197.

16. Prieto LT, Araújo CTP, Humel MMC, Souza EJ Jr, dos Santos Dias CT, Sartini Paulillo LAM. Influence of selective acid etching on microtensile bond strength of a self-adhesive resin cement to enamel and dentin. Braz J Oral Sci. 2010; 9:455–458.

17. De Munck J, Vargas M, Van Landuyt K, Hikita K, Lambrechts P, Van Meerbeek B. Bonding of an auto-adhesive luting material to enamel and dentin. Dent Mater. 2004; 20:963–971.

18. Hitz T, Stawarczyk B, Fischer J, Hämmerle CH, Sailer I. Are self-adhesive resin cements a valid alternative to conventional resin cements? A laboratory study of the long-term bond strength. Dent Mater. 2012; 28:1183–1190.

19. Sarr M, Mine A, De Munck J, Cardoso MV, Kane AW, Vreven J, Van Meerbeek B, Van Landuyt KL. Immediate bonding effectiveness of contemporary composite cements to dentin. Clin Oral Investig. 2010; 14:569–577.

20. Gerth HU, Dammaschke T, Züchner H, Schäfer E. Chemical analysis and bonding reaction of RelyX Unicem and Bifix composites--a comparative study. Dent Mater. 2006; 22:934–941.

21. Fu B, Sun X, Qian W, Shen Y, Chen R, Hannig M. Evidence of chemical bonding to hydroxyapatite by phosphoric acid esters. Biomaterials. 2005; 26:5104–5110.

22. Nagakane K, Yoshida Y, Hirata I, Fukuda R, Nakayama Y, Shirai K, Ogawa T, Suzuki K, Van Meerbeek B, Okazaki M. Analysis of chemical interaction of 4-MET with hydroxyapatite using XPS. Dent Mater J. 2006; 25:645–649.

23. Stawarczyk B, Hartmann R, Hartmann L, Roos M, Ozcan M, Sailer I, Hämmerle CH. The effect of dentin desensitizer on shear bond strength of conventional and self-adhesive resin luting cements after aging. Oper Dent. 2011; 36:492–501.

24. Hiraishi N, Yiu CK, King NM, Tay FR. Effect of 2% chlorhexidine on dentin microtensile bond strengths and nanoleakage of luting cements. J Dent. 2009; 37:440–448.

25. International Organization for Standardiation. ISO TR 11405. Dental material guidence on testing of adhesion to tooth structure. 1994.

26. Di Hipólito V, Rodrigues FP, Piveta FB, Azevedo Lda C, Bruschi Alonso RC, Silikas N, Carvalho RM, De Goes MF, Perlatti D'Alpino PH. Effectiveness of self-adhesive luting cements in bonding to chlorhexidine-treated dentin. Dent Mater. 2012; 28:495–501.

27. Akca T, Yazici AR, Celik C, Ozgünaltay G, Dayangaç B. The effect of desensitizing treatments on the bond strength of resin composite to dentin mediated by a self-etching primer. Oper Dent. 2007; 32:451–456.

28. Sailer I, Oendra AE, Stawarczyk B, Hämmerle CH. The effects of desensitizing resin, resin sealing, and provisional cement on the bond strength of dentin luted with self-adhesive and conventional resincements. J Prosthet Dent. 2012; 107:252–260.

29. Sailer I, Tettamanti S, Stawarczyk B, Fischer J, Hämmerle CH. In vitro study of the influence of dentin desensitizing and sealing on the shear bond strength of two universal resin cements. J Adhes Dent. 2010; 12:381–392.

30. Stawarczyk B, Hartmann L, Hartmann R, Roos M, Ender A, Ozcan M, Sailer I, Hämmerle CH. Impact of Gluma Desensitizer on the tensile strength of zirconia crowns bonded to dentin: an in vitro study. Clin Oral Investig. 2012; 16:201–213.

31. Munksgaard EC, Asmussen E. Bond strength between dentin and restorative resins mediated by mixtures of HEMA and glutaraldehyde. J Dent Res. 1984; 63:1087–1089.

32. Qin C, Xu J, Zhang Y. Spectroscopic investigation of the function of aqueous 2-hydroxyethylmethacrylate/glutaraldehyde solution as a dentin desensitizer. Eur J Oral Sci. 2006; 114:354–359.

33. Nakabayashi N, Watanabe A, Gendusa NJ. Dentin adhesion of "modified" 4-META/MMA-TBB resin: function of HEMA. Dent Mater. 1992; 8:259–264.

34. Arrais CA, Chan DC, Giannini M. Effects of desensitizing agents on dentinal tubule occlusion. J Appl Oral Sci. 2004; 12:144–148.

35. Kolker JL, Vargas MA, Armstrong SR, Dawson DV. Effect of desensitizing agents on dentin permeability and dentin tubule occlusion. J Adhes Dent. 2002; 4:211–221.

36. Dündar M, Cal E, Gökçe B, Türkün M, Ozcan M. Influence of fluoride- or triclosan-based desensitizing agents on adhesion of resin cements to dentin. Clin Oral Investig. 2010; 14:579–586.

37. Pashley EL, Tao L, Pashley DH. Effects of oxalate on dentin bonding. Am J Dent. 1993; 6:116–118.

38. De Andrade e Silva SM, Malacarne-Zanon J, Carvalho RM, Alves MC, De Goes MF, Anido-Anido A, Carrilho MR. Effect of oxalate desensitizer on the durability of resinbonded interfaces. Oper Dent. 2010; 35:610–617.

39. Pashley DH, Carvalho RM, Pereira JC, Villanueva R, Tay FR. The use of oxalate to reduce dentin permeability under adhesive restorations. Am J Dent. 2001; 14:89–94.

40. Tay FR, Pashley DH, Mak YF, Carvalho RM, Lai SC, Suh BI. Integrating oxalate desensitizers with total-etch two-step adhesive. J Dent Res. 2003; 82:703–707.

41. Aranha AC, Siqueira Junior Ade S, Cavalcante LM, Pimenta LA, Marchi GM. Microtensile bond strengths of composite to dentin treated with desensitizer products. J Adhes Dent. 2006; 8:85–90.

42. Pisani-Proença J, Erhardt MC, Amaral R, Valandro LF, Bottino MA, Del Castillo-Salmerón R. Influence of different surface conditioning protocols on microtensile bond strength of self-adhesive resin cements to dentin. J Prosthet Dent. 2011; 105:227–235.

43. Breschi L, Mazzoni A, Nato F, Carrilho M, Visintini E, Tjäderhane L, Ruggeri A Jr, Tay FR, Dorigo Ede S, Pashley DH. Chlorhexidine stabilizes the adhesive interface: a 2-year in vitro study. Dent Mater. 2010; 26:320–325.

44. Santos MJ, Bapoo H, Rizkalla AS, Santos GC. Effect of dentin-cleaning techniques on the shear bond strength of self-adhesive resin luting cement to dentin. Oper Dent. 2011; 36:512–520.

45. Lin J, Kern M, Ge J, Zhu J, Wang H, Vollrath O, Mehl C. Influence of peripheral enamel bonding and chlorhexidine pretreatment on resin bonding to dentin. J Adhes Dent. 2013; 15:351–359.

46. Al-Saud LM, Al-Nahedh HN. Occluding effect of Nd:YAG laser and different dentin desensitizing agents on human dentinal tubules in vitro: a scanning electron microscopy investigation. Oper Dent. 2012; 37:340–355.

47. Kumar NG, Mehta DS. Short-term assessment of the Nd:YAG laser with and without sodium fluoride varnish in the treatment of dentin hypersensitivity--a clinical and scanning electron microscopy study. J Periodontol. 2005; 76:1140–1147.

48. de Oliveira MT, de Freitas PM, de Paula Eduardo C, Ambrosano GM, Giannini M. Influence of Diamond Sono-Abrasion, Air-Abrasion and Er:YAG Laser Irradiation on Bonding of Different Adhesive Systems to Dentin. Eur J Dent. 2007; 1:158–166.

49. RelyX™ U200. Self-Adhesive Resin Cement, Technical Data Sheet. St. Paul; MN; USA: 3M ESPE.