Korean J Dermatol.  1990 Jun;28(3):308-314.

Immunohistochemical Staining of Amyloid Deposit with Monoclonal Anti - keratin Antibodies in Primary Localized Cutaneous Amyloidosis

• Cho KH
• Chang SH
• Kim JA
• Lee YS
• Ham EK

Abstract

Nine cases of primary localized cutaneous amyloidosis were studied by immunoperoxidase technique (ABC method) employing anti-keratin antibodies. All specimens were examined using consecutive paraffin sections to confirm the correspondence between amyloid existing area and reactive sites. Anti-keratin antibody 34pE which recognize 68, 58, 56.5, 56kd keratin peptides reacted with amyloid deposits in both lichen amyloidosus and macular amyloidosis. However, anti-keratin antibodies 34pB4 and 35pH did not react with amyloids. In general, Dylon staining positive material, keratin reacted with 34pE and amyloid P showed similar distribution in serial sections, but did not show the same one. Several keratin bodies reacted with 34pE, which were not stained with Dylon staining or antiamyloid P were found in the dermis of one specimen. These results suggest that immunohistochemical staining with antikeratin antibody 34pE using formalin-fixed, paraffin-embedded sections appeared to be a useful method in studying the histogenesis of primary localized cutaneous arnyloidosis.

Keyword

Amyloid P; Cutaneous amyloidosis; Keratin

MeSH Terms

Amyloid*
Amyloidosis*
Antibodies*
Catalytic Domain
Dermis
Immunoenzyme Techniques
Lichens
Paraffin
Peptides
Plaque, Amyloid*
Amyloid
Antibodies
Paraffin
Peptides