Korean Circ J.  1988 Mar;18(1):69-83. 10.4070/kcj.1988.18.1.69.

Electron Microscopic Study on Mitochondria and Cytochrome Oxidase Activity Following Acute Carbon Monoxide Exposure in Rat Myocardium

• Chong DY
• Lee Y
• Cho SH
• Song KY

• KMID: 2229593
• DOI: http://doi.org/10.4070/kcj.1988.18.1.69

Abstract

The problem of carbon monoxide intoxication has been increasing since 1950s and it is still regarded as one of the most serious public health problem in Korea. If the patients survives the acute episode, elimination of carbon monoxide occurs in the rate of a halt-time of 3 to 4 hours. Nevertheless, there may remain after effect. Therefore, the present study was desinged to elucidate the change of myocardial tissue caused by acute carbon monoxide poisoning were evaluated by electrton microscopic observation of the severity of the mitochondrial swelling and cytochrome oxidase activity, which was demonstrated by histochemical staining with diaminobenzidine(DAB). Male Sprague-Dawley rats were exposed to the carbon monoxide of 1500 ppm for 150 minutes. They were divided into two groups ; one group were treated with hyperbaric oxygenation 15 minutes after carbon monoxide exposure, the other were not treated. Rats were sacrified 6 hours, 12 hours, 1 day, 3 days, 7days, 14 days, and 21 days after carbon monoxide exposure. The specimens were collected from the apex of the heart. In the group without hyperbaric oxygenation, mitochondrial swelling subsided 21 days after carbon monoxide exposure accompained by restoration of cytochrome oxidase activity. On the other hand, with hyperbaric oxygenation, healing process was shortened. Cytochrome oxidase activity was restored 7 days after carbon monoxide exposure.

Keyword

Carbon monoxide intoxication; Myocardiium; Cytochrome oxidase; Hyperbaric Oxygenation

MeSH Terms

Animals
Carbon Monoxide Poisoning
Carbon Monoxide*
Carbon*
Cytochromes*
Electron Transport Complex IV*
Hand
Heart
Humans
Hyperbaric Oxygenation
Korea
Male
Mitochondria*
Mitochondrial Swelling
Myocardium*
Public Health
Rats*
Rats, Sprague-Dawley
Carbon
Carbon Monoxide
Cytochromes
Electron Transport Complex IV