Korean Circ J.  1991 Aug;21(4):693-699. 10.4070/kcj.1991.21.4.693.

In Vivo Delineation of Regional Myocardial Perfusion in Open-Chest Dog by Hydrogen Peroxide Myocardial Contrast Echocardiography

• Kim HK
• Kim YH
• Ahn TH
• Suh HS
• Ro YM

• KMID: 2229158
• DOI: http://doi.org/10.4070/kcj.1991.21.4.693

Abstract

Intravascular injection of hydrogen peroxide produces oxygen microbubbles suitable for echocardiographic contrast enhancement. To evaluate the effect of a method of myocardial contrast 2-D-echocardiographic delineation of myocardium during acute coronary occlusion, injection of a fresh mixture of 2ml of 0.2% H2O2 and 1ml of heparinized dog blood into aortic root were made in 12 poenchest dogs 10 minutes after occlusion of left anterior descending coronary artery distal to the first diagonal branch and left ventricular short axis 2-D echocardiographic images at the midpapillary muscle level were obtained. On injection of H2O2 blood mixture normally perfused myocardium was enhanced in echodensity but the area of malperfusion did not change in echodensity. The borderlines between the area of normal perfusion and malperfusion was well delineated. The malperfused area measured at mid papillary muscle level by planimetry area method was 29.7+/-6.0% and 32.6+/-6.7% by endocardial circumferential length method. There was a linear correlation between planimetric estimate of area of malperfusion by H2O2 contrast echocardiography and visual determination of regional wall motion abnormality by 2-D echocardiography(r=0.93, P<0.001). There was no change in heart rate before, during and after H2O2 injection. Infection of H2O2 blood mixture caused bradycardia(8.3%), second degree A-V block(16.6%) and ventricular fibrillation(8.3%). H2O2 clearance was achieved in 3-10 minutes. These findigs suggest that H2O2 enhanced myocardial contrast ehocargiography using 2ml of 0.2% H2O2 and 1ml of blood muxture is an accurate, reproducible, real-time in vivo method of quantifying the extent of myocardial perfusion defect during acute coronary occlusion in dog.

MeSH Terms

Animals
Axis, Cervical Vertebra
Coronary Occlusion
Coronary Vessels
Dogs*
Echocardiography*
Heart Rate
Heparin
Hydrogen Peroxide*
Hydrogen*
Microbubbles
Myocardium
Oxygen
Papillary Muscles
Perfusion*
Heparin
Hydrogen
Hydrogen Peroxide
Oxygen