Korean J Androl.  2011 Aug;29(2):127-133. 10.5534/kja.2011.29.2.127.

The Effect of Cyanidin-3-O-beta-d-glucopyranoside on the Penile Erection and Corpus Cavernosum in a Rat Model of Diabetic Erectile Dysfunction

• Ha US
• Koh JS
• Woo JC
• Kim SJ
• Kim SJ
• Jang H
• Yoon BI
• Hwang SY
• Kim SW

• KMID: 2226465
• DOI: http://doi.org/10.5534/kja.2011.29.2.127

Abstract

PURPOSE
The aim of this study was to evaluate Cyanidin-3-O-beta-d-glucopyranoside on improvement and protection for erectile function.
MATERIALS AND METHODS
Sprague-Dawley rats (12wks old) were divided into three groups (n=12 in each): normal control, diabetes (DM), and diabetes with Cyanidin-3-O-beta-d-glucopyranoside (C3G) concentration materials treatment (DM+C3G). DM and DM+C3G group received a single injection of streptozotocin (50 mg/kg), and 4 wk after induction of diabetes, DM+C3G group were treated with daily C3G (10 mg/kg) dissolved in water for 8 wk. After 12 wk of streptozotocin injections, rats in each group underwent intracavernosal pressure measurement (ICP) and then the corporal tissues were sampled.
RESULTS
DM group showed markedly lower erectile parameters than those in the control group, whereas rats in the DM+C3G group showed improved erectile function by minimizing corporal apoptosis.
CONCLUSIONS
The current study is the first to suggest that Cyanidin-3-O-beta-d-glucopyranoside may have a potency to improve and protect erectile function in a rat model of diabetic erectile dysfunction.

Keyword

Cyanidin-3-O-beta-d-glucopyranoside; Diabetes Mellitus; Erectile dysfunction

MeSH Terms

Animals
Diabetes Mellitus
Erectile Dysfunction
Male
Penile Erection
Rats
Rats, Sprague-Dawley
Streptozocin
Water
Streptozocin
Water

Figure

• 

  Fig. 1. Masson's trichrome staining for collagen (blue) and smooth muscle (red) in corporal tissue of the control (A), diabetes (B), and diabetes treated with C3G (C) groups. ×200.

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  Fig. 2. Immunohistochemical in situ TUNEL detection of apoptosis in corporal tissue of the control (A), diabetes (B), and diabetes treated with C3G (C) groups. Cells undergoing apoptosis, called apoptotic bodies, show as black or dark brown in the TUNEL assay, while living cells are shown as lighter dots. ×400.

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