Korean J Clin Pathol.
2000 Oct;20(5):510-515.
Reactivities of Commercial Antisera for HLA Typing
- Affiliations
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- 1Department of Clinical Pathology, Yonsei University College of Medicine, Seoul, Korea.
- 2Department of Transplant Surgery, Yonsei University College of Medicine, Seoul, Korea.
Abstract
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BACKGROUND: The most common problem in HLA typing is unsatisfactory quality of the antisera, or a lack of understanding of their reactivities. Therefore, commercial antisera must be verified under the conditions applied in a particular tissue typing laboratory.
METHODS
We evaluated the antisera reactivities of a commercial HLA-yping tray, Lymphotype HLA-BC 72 oriental, the lot 7220999, 7230100 (Biotest, Germany), in about 300 samples from organ transplant recipients and healthy potential donors.
RESULTS
The relatively weak antisera were those that defined A26, A33, Cw5, Cw14, B46, B58, B64 and B71 etc. Some of these antisera were not indicated as 'weak reaction' in the test result catalogue. The reactivities of each antisera indicated as 'extra reaction' or 'sometimes missing' were various.
CONCLUSIONS
As for antisera reactivities, the data obtained by a laboratory itself are necessary in addition to those in the test result catalogue. These data will be helpful for the correct interpretation for laboratories using same commercial kits.
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