J Korean Surg Soc.  2012 Feb;82(2):70-78. 10.4174/jkss.2012.82.2.70.

Local tissue reaction after injection of contrast media on gastric wall of mouse: experimental study for application of contrast media to computed tomography lymphography

• Hwang SH
• Kim HH
• Park DJ
• Jee YS
• Lee KH
• Kim YH
• Lee HS
• Lee HJ
• Yang HK

• KMID: 2212204
• DOI: http://doi.org/10.4174/jkss.2012.82.2.70

Abstract

PURPOSE
Computed tomography (CT) lymphography is a simple technique of sentinel node navigation but tissue reaction after injection of contrast media has not been reported yet.
METHODS
Ninety mice used in this study were divided into three groups: lipiodol, iopamidol, and normal saline. The test compounds were given by submucosal injection to the gastric wall of anesthetized mice. The specimens were subjected to histopathological examination.
RESULTS
The mean grades of acute inflammatory response after iopamidol and lipiodol injection were significantly higher than control group. However, there was no significant difference between iopamidol and lipiodol injection. The mean grade of chronic inflammatory response and fibrosis showed no differences between groups. The presence or absence of fibrinoid necrosis and mesothelial hyperplasia showed no statistical differences at each time point between groups. The foam cell, which is similar to human signet ring cell carcinoma, were not identified in normal saline and iopamidol group, but were detected by postoperative day 7 in lipiodol group.
CONCLUSION
We conclude that iopamidol and lipiodol when used as a contrast media of CT lymphography is an available material for preoperative sentinel node navigation surgery for gastric cancer with an acceptable incidence of pathological alterations in a mouse model. Our results are potentially useful to clinical (human) application.

Keyword

Lymphography; Sentinel node; Tissue reaction; Contrast media

MeSH Terms

Animals
Carcinoma, Signet Ring Cell
Contrast Media
Ethiodized Oil
Fibrosis
Foam Cells
Humans
Hyperplasia
Incidence
Iopamidol
Lymphography
Mice
Necrosis
Nitriles
Pyrethrins
Stomach Neoplasms
Contrast Media
Ethiodized Oil
Iopamidol
Nitriles
Pyrethrins

Figure

• 

  Fig. 1 (A-D) Grade of acute inflammation. (E, F) Grade of chronic inflammation. (G-J) Grade of fibrosis. (K) Presence of foam cell. (L) Presence of fibrinoid necrosis. (M) Presence of mesothelial hyperplasia. A, D, E, G-J, L (H&E, ×40), B, C, F, K, M (H&E, ×100).

• 

  Fig. 2 Comparisons of acute inflammatory response between groups.

• 

  Fig. 3 Comparisons of chronic inflammatory response between groups.

• 

  Fig. 4 Comparisons of fibrosis between groups.

• 

  Fig. 5 (A) Large histiocytic cells showing foamy cytoplasm in murine gastric mucosa after injection of lipiodol (post-operative 3 days). (B) Human signet ring cell carcinoma (A, H&E, ×200, B, H&E, ×400).

• 

  Fig. 6 Uptake of contrast agent on radiologic examination. The circled part of each figure shows the uptake of contrast agent. The uptake of iopamidol at postoperative day (POD) 1 is identified in Fig. 6A. Fig. 6B-D also show lipiodol uptake at POD 1, 3, and 7, respectively.

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