J Korean Ophthalmol Soc.  2002 Dec;43(12):2555-2564.

Preretinal Neovascularization by Laser-induced Thrombosis in Albino Rats and Inhibition of Neovascularization by Genistein

• Park KH
• Lee JJ
• Kang SG
• Lee JK
• Lee SM
• Kim JH
• Yu YS
• Lee JH
• Chung H

Abstract

PURPOSE: To determine the effect of genistein, an inhibitor of protein tyrosine kinase, on preretinal neovascularization through the quantification of retinal neovascularization using image analyzer in an experimental rat model.
METHODS
In 36 eyes of 36 rats, retinal vein occlusion was induced by photodynamic therapy with an argon green laser and systemic injection of rose bengal (40 mg/kg). The development and progression of retinal neovascularization was followed weekly by fluorescein angiography. Seven rats were sacrificed each week, after which two eyes were prepared with H and E staining for histologic examination, and five were prepared as a control group using ADPase staining for neovascularization analysis. In the remaining fifteen eyes, retinal vein occlusion was also induced using the same method. Immediately after vein occlusion, 4.0 mg of genistein dissolved in dimethyl sulfoxide (DMSO) was injected intraperitoneally twice a day for the first 7 days. Five rats were sacrificed each week and stained with ADPase. After ADPase staining, those samples with evidence of neovascularization were quantified using an image analyzer.
RESULTS
No retinal neovasularizaion was found at the end of the first week. The size of retinal neovascularization for the five eyes sacrificed at the end of week 2 and 3 were 6.53+/-2.11 mm2 and 3.77+/-3.51 mm2 in the control group, and 2.22+/-1.01 mm2 and 1.64+/-0.88 mm2 in the genistein treatment group, respectively. Retinal neovascularization was successfully suppressed until two weeks after laser treatment by genistein in this rat neovascularization model.
CONCLUSIONS
Genistein may be a useful treatment modality to suppress retinal neovascularization complicated with retinal ischemic injury.

Keyword

Neovascularization; Photodynamic therapy; ADPase staining; Genistein

MeSH Terms

Animals
Apyrase
Argon
Dimethyl Sulfoxide
Fluorescein Angiography
Genistein*
Models, Animal
Photochemotherapy
Protein-Tyrosine Kinases
Rats*
Retinal Neovascularization
Retinal Vein Occlusion
Retinaldehyde
Rose Bengal
Thrombosis*
Veins
Apyrase
Argon
Dimethyl Sulfoxide
Genistein
Protein-Tyrosine Kinases
Retinaldehyde
Rose Bengal