J Korean Soc Transplant.  2007 Dec;21(2):203-209.

A Study of Serum HSP in Heart Transplanted Rats

• Lee HY
• Kim JW
• Yoo DW
• Yoo CH
• Son SH

Abstract

PURPOSE: This study was performed to investigate the availability of the serum HSP72 and HSP27 as serologic markers of cardiac allograft rejection through rat heterotopic heart transplatation model.
METHODS
Inbred Lewis rats were randomly divided into three groups: the allograft heart transplant group, the isograft heart transplant group, and the sham-operated group. Six animals were studied in each group. In allograft heart tranplant group, the Brown Norway rats were used as donors and in isograft heart tranplant group, the Lewis rats were used as donors. The sera of the allograft heart transplanted rats, isograft heart transplanted rats, and sham- operated rats were collected at preoperative time, 3 days after operation and 6 days after operation, and analyzed for HSP72 and HSP27 by Western blots. Quantifications of band densities were carried out by laser densitometer and the results were expressed as % preoperative densities.
RESULTS
The levels of serum HSP72 of 3 days and 6 days after heart transplantation significantly increased in the allograft heart transplant group than in the isograft heart transplant group, respectively (160.2+/-44.8% vs. 109.0+/-34.7%, 276.0+/-72.1% vs. 175.0+/-44.2%, P<0.05). The levels of seum HSP27 of 3 days and 6 days after heart transplantation significantly increased in the allograft heart transplant group than in the isograft heart transplant group, respectively (162.3+/-62.7% vs. 118.4+/-37.0%, 235.7+/-67.1% vs. 127.9+/-40.8%, P<0.05).
CONCLUSION
It is concluded that serum HSP72 and HSP27 are useful markers to detect the cardiac allograft rejection.

Keyword

Heat shock protein; Transplantation; Heart

MeSH Terms

Allografts
Animals
Blotting, Western
Heart Transplantation
Heart*
Heat-Shock Proteins
Humans
Isografts
Norway
Rats*
Rats, Inbred Lew
Tissue Donors
Transplantation
Heat-Shock Proteins