J Korean Acad Prosthodont.  2011 Oct;49(4):333-340. 10.4047/jkap.2011.49.4.333.

The effect of different crystallization temperature of the hydroxyapatite coating produced by ion beam-assisted deposition on anodizing-treated titanium disks on human osteosarcoma cells

• Pae A
• Won HD
• Leesungbok R
• Kim HS
• Woo YH

• KMID: 2195528
• DOI: http://doi.org/10.4047/jkap.2011.49.4.333

Abstract

PURPOSE
The aim of this study was to study the effect of hydroxyapatite (HA) coating crystallinity on the proliferation and differentiation of human osteosarcoma cells.
MATERIALS AND METHODS
Surface roughness of the titanium disks increased by anodizing treatment and then HA was coated using ion beam-assisted deposition (IBAD). HA coating was crystallized by heat-treated at different temperature (100degrees C, 300degrees C, 500degrees C, 800degrees C). According to the temperature, disks were divided into four groups (HA100, HA300, HA500, HA800). With the temperature, crystallinity of the HA coating was different. Anodized disks were used as control group. The physical properties of the disk surface were evaluated by surface roughness tests, XRD tests and SEM. The effect of the crystallinity of HA coating on HOS cells was studied in proliferation and differentiation. HOS cells were cultured on the disks and evaluated after 1, 3, 5, and 7 days. Growth and differentiation kinetics were subsequently investigated by evaluating cell proliferation and alkaline phosphatase activity.
RESULTS
Regardless of the heat-treated temperature, there is no difference on the surface roughness. Crystallinity of the HA was appeared in the groups of HA500, HA800. HOS cells proliferation, ALP activity were higher in HA500 and HA800 group than HA100 and HA300.
CONCLUSION
Within the results of this limited study, heat treatment at 500degrees C of HA coating produced by IBAD has shown greater effect on proliferation and differentiation of HOS cells. It is considered that further in vivo study will be necessary.

Keyword

Cell proliferation; Alkaline phophatase activity

MeSH Terms

Alkaline Phosphatase
Cell Proliferation
Crystallins
Crystallization
Durapatite
Hot Temperature
Humans
Kinetics
Osteosarcoma
Titanium
Alkaline Phosphatase
Crystallins
Durapatite
Titanium

Figure

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  Fig. 1. SEM morphologies (×2,000). A: anodizing treated (control group), B: HA100, C: HA300, D: HA500, E: HA800 group shows some cracks (indicated arrows).

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  Fig. 2. The results of X-ray diffraction (XRD) test. At HA500 and HA800 group, HA peak appeared but there is no appearance of HA peak at HA100 and HA300 group. A: control group, B: HA100, C: HA300, D: HA500, E: HA800.

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