J Korean Fract Soc.  2009 Jan;22(1):45-50. 10.12671/jkfs.2009.22.1.45.

The Effect of COX-2 Inhibitor on the Expression of MMP-13 during Early Fracture Healing Phase in Rats

  • 1Department of Orthopaedic Surgery, Yong-San Hospital, Chung-Ang University College of Medicine, Seoul, Korea. gustinolhj@hanafos.com


This study investigated the effect of COX-2 inhibitor on the expression of MMP-13 in the healing process of fracture. MATERIAL AND METHODS: Adult Sprague-Dawley rats were divided into two groups of twenty five rats each. Unilateral femoral shaft fractures were created artificially under displacement in all two groups. COX-2 inhibitor was only given to the experimental group from the postoperative day 1. At 2 weeks after fracture the rats were sacrificed and the callus from each group was used for histologic examination and real time RT-PCR for MMP-13 expression.
Histologically, proliferation of osteoblasts and formation of osteoid was less abundant in the experimental group. In real time RT-PCR, the mean expression of MMP-13 is 2.84+/-2.50 in the control group compared with 1.16+/-1.05 in the experimental group.
In the early stage of fracture healing, COX-2 inhibitor suppress the expression of MMP-13.


Fracture; Healing; MMP-13; COX-2 Inhibitor

MeSH Terms

Bony Callus
Displacement (Psychology)
Fracture Healing
Rats, Sprague-Dawley


  • Fig. 1 This radiograph shows experimental fracture with Intramedullary nailing of rat femur.

  • Fig. 2 The histologic sections of calluses show that the experimental group has significantly more fibrous tissue and less new bone formation than the control group. (A) Standard group (×100 H&E Stain). (B) Experimental group (×100 H&E Stain).


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