J Korean Acad Conserv Dent.  2003 May;28(3):241-248. 10.5395/JKACD.2003.28.3.241.

PCR-based Identification of Eubacteirum species in endodontic infection

Affiliations
  • 1Yonsei Dental College, Department of Conservative Dentistry, Korea. kum6139@yumc.yonsei.ac.kr
  • 2Oral Science Research Center, UCHC Endo, CT, USA.

Abstract

No abstract available.


Figure

  • Fig. 1 Phylogenetic analysis of 16S rRNA gene sequences with oral asaccharolytic Eubacterium-branch specific primer 557R and universal primer 27F, sequences of oral asaccharolytic Eubacterium species and most closely related species retrieved from sequence databases.


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Reference

1. Wade WG, Lewis MA, Cheeseman SL, Absi EG, Bishop PA. An unclassified Eubacterium taxon in acute dento-alveolar abscess. J Med Microbiol. 1994. 40:115–117.
2. Wade WG. The role of Eubacterium species in periodontal disease and other oral infections. Microb Ecol Health Dis. 1996. 9:367–370.
Article
3. Sundqvist G. Associations between microbial species in dental root canal infections. Oral Microbiol Immunol. 1992. 7:257–262.
Article
4. Wasfy MO, McMahon KT, Minah GE, Falkler WA Jr. Microbiological evaluation of periapical infections in Egypt. Oral Microbiol Immunol. 1992. 7:100–105.
Article
5. Yoshida M, Fukushima H, Yamamoto K, Ogawa K, Toda T, Sagawa H. Correlation between clinical symptoms and microorganisms isolated from root canals of teeth with periapical pathosis. J Endod. 1987. 13:24–28.
Article
6. Sundqvist G, Johansson E, Sjogren U. Prevalence of black-pigmented bacteroides species in root canal infections. J Endod. 1989. 15:13–19.
Article
7. Sjögren U, Figdor D, Persson S, Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J. 1997. 30:297–306.
Article
8. Kageyama A, Benno Y, Nakase T. Phylogenetic evidence for the transfer of Eubacterium lentum to the genus Eggerthella as Eggerthella lenta gen. nov., comb. nov. Int J Syst Bacteriol. 1999. 49:1725–1732.
Article
9. Fouad AF, Barry J, Caimano M, Clawson C, Zhu Q, Carver R, Hazlett K, Radolf JD. PCR-based identification of bacteria in endodontic infections. J Clin Microbiol. 2002. 40:3223–3231.
Article
10. Rolph HJ, Lennon A, Riggio MP, Saunders WP, MacKenzie D, Coldero L, Bagg J. Molecular identification of microorganisms from endodontic infections. J Clin Microbiol. 2001. 39:3282–3289.
Article
11. Conrads G, Soffner J, Pelz K, Mutters R. Taxonomic update and clinical significance of species within the genus Peptostreptococcus. Clin Infect Dis. 1997. 25:S94–S97.
12. Felsenstein J. PHYLIP (Phylogeny Inference Package) version 3.5c. 1993. Seattle: Department of Genetics, University of Washington;Distributed by the author.
13. Page RD. TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci. 1996. 12:357–358.
Article
14. Socransky SS, Haffajee AD, Dzink JL, Hillman JD. Associations. between microbial species in subgingival plaque samples. Oral Microbiol Immunol. 1988. 3:1–7.
Article
15. Spratt DA, Weightman AJ, Wade WG. Diversity of oral asaccharolytic Eubacterium species in periodontitis--identification of novel phylotypes representing uncultivated taxa. Oral Microbiol Immunol. 1999. 14:56–59.
Article
16. Nakazawa F, Sato M, Poco SE, Hashimura T, Ikeda T, Kalfas S, Sundqvist G, Hoshino E. Description of Mogibacterium pumilum gen. nov., sp. nov. and Mogibacterium vescum gen. nov., sp. nov., and reclassification of Eubacterium timidum (Holdeman et al. 1980) as Mogibacterium timidum gen. nov., comb. nov. Int J Syst Evol Microbiol. 2000. 50(Pt 2):679–688.
Article
17. Haapasalo M, Kerosuo E, Lounatmaa K. Hydrophobicities of human PMN leukocytes and oral Bacteroides and Porphyromonas spp., Wolinella recta, and E. yurii with special reference to bacterial surface structures. Scand J Dent Res. 1990. 98:472–481.
Article
18. Downes J, Munson MA, Spratt DA, Kononen E, Tarkka E, Jousimies-Somer H, Wade WG. Characterization of Eubacterium-like strains isolated from oral infections. J Med Microbiol. 2001. 50:947–951.
Article
19. Cheeseman SL, Hiom SJ, Weightman AJ, Wade WG. Phylogeny of oral asaccarolytic Eubacterium species determined by 16S rRNA sequence comparision and proposal of Eubacterium infirmum sp.nov. and Eubacterium tardum sp.nov. Int J Syst Bacteriol. 1996. 46(4):957–959.
Article
20. Hashimura T, Sato M, Hoshino E. Detection of Slackia exigua, Mogibacterium timidum and Eubacterium saphenum from pulpal and periradicular samples using the Polymerase Chain Reaction (PCR) method. Int Endod J. 2001. 34:463–470.
Article
21. Smith AJ, Wade WG. Serum antibody response against oral Eubacterium species. J Periodontal Res. 1999. 34:175–178.
Article
22. Fouad AF, Burleson J. The effct of diabetes mellitus on endodontic treatment outcome. J Am Dent Assoc. 2003. 134:43–51.
23. Wade WG, Downes J, Dymock D, Hiom SJ, Weightman AJ, Dewhirst FE, Paster BJ, Tzellas N, Coleman B. The family Coriobacteriaceae: reclassification of Eubacterium exiguum (Poco et al. 1996) and Peptostreptococcus heliotrinreducens (Lanigan 1976) as Slackia exigua gen. nov., comb. nov. and Slackia heliotrinireducens gen. nov., comb. nov., and Eubacterium lentum (Prevot 1938) as Eggerthella lenta gen. nov., comb. nov. Int J Syst Bacteriol. 1999. 49:595–600.
Article
24. Wade WG, Slayne MA, Aldred MJ. Comparison of identification methods for oral asaccharolytic Eubacterium species. J Med Microbiol. 1990. 33:239–242.
Article
25. Wade WG. The role of Eubacterium species in periodontal disease and other oral infections. Microb Ecol Health Dis. 1997. 9:367–370.
Article
26. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S rDNA amplification for phylogenetic study. J Bacteriol. 1991. 173:697–703.
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