Korean J Med.
2006 Aug;71(2):189-197.
Evaluation of repetitive sequence-based genomic fingerprinting for molecular classification and identification of vibrio species
- Affiliations
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- 1Department of Microbiology Chung-Ang University College of Medicine1 Seoul, Korea. sichung@cau.ac.kr
- 2Department of Internal Medicine, Hansol Hospital, Seoul, Korea.
- 3Department of Pediatrics, Medical College of Konkuk University, Chungju, Korea.
Abstract
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BACKGROUND: The aims of this study were to compare the suitability of repetitive-PCR genomic fingerprinting procedures to investigate genetic relatedness of the genus Vibrio and its applicability for the molecular identification of Vibrio vulnificus.
METHODS
Forty-eight Vibrio strains were included for this study. REP-, ERIC-, BOX- and SERE-PCR were compared with 13 members of the genus Vibrio.
RESULTS
REP-, BOX- and SERE-PCR showed V. vulnificus strains could not be separated well from other Vibrio species. However, approximately 320 bp of highly discriminatory specific fragments was recovered from V. vulnificus strains by ERIC-PCR.
CONCLUSIONS
ERIC-PCR could be used as rapid classification and identification methods of V. vulnificus from other members of the genus Vibrio.
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