J Biomed Res.
2014 Dec;15(4):176-181. 10.12729/jbr.2014.15.4.176.
Stimulation of keratinocyte differentiation by extract of combined medicinal plant
- Affiliations
-
- 1Department of Dermatology, First Affiliated Hospital of Dalian Medical University, Dalian, China.
- 2Department of Dermatology, School of Medicine, Chungnam National University, Daejeon 301-747, Korea. jhoon@cnu.ac.kr
- 3Department of Obstetrics & Gynecology, College of Korean Medicine, Dongguk University, Seoul 100-715, Korea.
- KMID: 2168996
- DOI: http://doi.org/10.12729/jbr.2014.15.4.176
Abstract
- Skin is the outermost organ and acts as a barrier between the organism and environment. Skin protects the organism from environmental insults, such as chemicals, pathogens, and UV light. Much of the protective function of skin is dependent on the epidermis, a multi-layered epithelium that is composed of various cell types such as keratinocytes and melanocytes. Keratinocytes produce protective components through a sophisticated differentiation process. Disturbance of keratinocyte differentiation is related to several skin diseases such as psoriasis and atopic dermatitis. In this study, we prepared extract of combined medicinal plants (ECMP) consisting of Taraxacum platycarpum H. Dahlstedt, Heartleaf Houttuynia, Glycyrrhiza uralensis Fischer, and root bark of Ulmus davidiana. We demonstrated that ECMP enhanced keratinocyte differentiation and barrier functionality using an in vitro cell culture system and in vivo animal test. Treatment of cultured keratinocytes with ECMP resulted in induction of keratinocyte differentiation, as evidenced by increased differentiation markers such as involucrin, loricrin, and filaggrin. In line with these results, ECMP decreased proliferation of keratinocytes cultured in vitro. ECMP applied topically to tape-stripped mouse skins accelerated reduction of transepidermal water loss (TEWL), indicating fast recovery of barrier function. Immunohistochemistry showed that ECMP increased the filaggrin level in tape-stripped mouse skins. These results suggest that ECMP may be applicable for keratinocyte differentiation-related skin diseases.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Effect of medicinal plant extract for hangover relief
- Studies on anti-oral cancer activities of medicinal plant extracts
- Ovicidal and Oviposition Deterrent Activities of Medicinal Plant Extracts Against
Aedes aegypti L. andCulex quinquefasciatus Say Mosquitoes (Diptera: Culicidae) - Treatment of natural tropical theileriosis with the extract of the plant Peganum harmala
- The Efficacy and Safety of the Topical Application of Poly-Gamma Glutamic Acid Hydrogel Nanoparticle-Based Fermented Extract of a Medicinal Plant Solution for Androgenetic Alopecia: A Randomized Double-Blinded Placebo-Controlled Clinical Trial
