J Bacteriol Virol.  2012 Dec;42(4):339-345. 10.4167/jbv.2012.42.4.339.

Detection of Adeno-associated Virus from Semen Suffering with Male Factor Infertility and Having Their Conception Partners with Recurrent Miscarriages

• Kim CH
• Kim JH
• Kim HJ
• Kim KW
• Lee JY
• Yang SH
• Choe J
• Hwang D
• Kim KC
• Hwang ES

• KMID: 2168669
• DOI: http://doi.org/10.4167/jbv.2012.42.4.339

Abstract

Adeno-associated virus (AAV) and human papillomavirus (HPV) DNAs were found in abnormal quality semen, early abortus and female genital tissues. It was suggested that they might cause male infertility and miscarriages. This study was performed to determine the detection rate of these viruses in the semen and to assess the relationship between the presence of virus and male factor infertility and recurrent miscarriages. Sixty-three of 99 recruited male were included in this study according to the completeness of follow-up and the sample availability. Fourteen male with normal reproductive capacity were allocated to control group, 15 male with abnormal results in semen analysis were grouped as male factor infertility (MF) group, and 34 male whose spouses have had history of repeated spontaneous abortions were designated as repeated miscarriage (RM) group. AAV and HPV were detected in semen by polymerase chain reaction. The detection rate of AAV in the MF infertility group and RM group was 60.0% and 50.0%, respectively, while 14.3% in the control group (p < 0.05). However, the differences in the detection rate of HPV were not statistically significant among groups. These results suggest that AAV could be related to repeated miscarriages and male infertility.

Keyword

Adeno-associated virus; Human papilloma virus; Semen; Male factor; Repeated miscarriage

MeSH Terms

Abortion, Habitual
Abortion, Spontaneous
Dependovirus
DNA
Female
Fertilization
Follow-Up Studies
Humans
Infertility
Infertility, Male
Male
Polymerase Chain Reaction
Pregnancy
Semen
Semen Analysis
Spouses
Stress, Psychological
Viruses
DNA

Figure

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  Figure 1 Gel electrophoresis analysis of PCR products from positive controls. M) 100 bp DNA ladder size marker, (A) Achondroplasia (179 bp), (B) primary PCR for AAV2 (AUS1-Pan3 427 bp), (C) nested PCR for AAV2 (IN1-IN2 150 bp), (D) HPV16 (~450 bp), (E) HPV18 (~450 bp). Electrophoresis was performed on a 2% agarose gel contained EtBr, and DNA bands were visualized under UV light.

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