J Bacteriol Virol.  2010 Dec;40(4):191-198. 10.4167/jbv.2010.40.4.191.

Genetic Diversity of Echovirus 6 Strains Circulating in Korea

• Jung E
• Park K
• Baek K
• Kim D
• Kang SY
• Kang B
• Cheon DS

• KMID: 2168583
• DOI: http://doi.org/10.4167/jbv.2010.40.4.191

Abstract

Echovirus 6 (ECV6) is the prevalent serotype detected in aseptic meningitis cases in Korea. To analyze the genetic variation of ECV6 isolates recently circulating in Korea, we determined the partial sequence of the VP1 capsid gene from 22 Korean ECV6 isolates and performed pairwise analysis against 42 reference strains from the GenBank database using MegAlign. The 22 Korean ECV6 isolates formed 3 distinct genetic clusters: Kor-lineage I, II, and III. The Korean ECV6 strains showed significant genetic diversity with 14.8~22.8% nucleotide divergence among the 3 different lineages. These ECV6 Kor-lineages were demonstrated to belong to different genetic clusters using VP1 sequence-based phylogenetic analysis, implying that the recently circulating Korean ECV6 strains have potential antigenic variation.

Keyword

Echovirus 6; VP1 capsid gene; Phylogenetic analysis

MeSH Terms

Antigenic Variation
Capsid
Databases, Nucleic Acid
Echovirus 6, Human
Enterovirus B, Human
Genetic Variation
Korea
Meningitis, Aseptic

Figure

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  Figure 1. Gel electrophoresis of PCR products of VP1 gene of 22 ECV6 isolates from patients with aseptic meningitis; Lane M, 100 bp DNA ladder; Lane 1-22, 22 ECV6 isolates; Lane N, negative control; Lane P, positive control.

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  Figure 2. Phylogenetic analysis based on a 286 bp sequence of the VP1 region of Korean ECV6 isolates. Nucleotide sequences were analyzed using the neighbor-joining method.

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  Figure 3. Phylogenetic analysis based on a 286 bp sequence of the VP1 of ECV6 isolates. Nucleotide sequences were analyzed by the neighbor-joining method. The numbers at the branches indicate the bootstrap values for 1,000 replicates.

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