Immune Netw.  2011 Oct;11(5):253-257. 10.4110/in.2011.11.5.253.

Vitamin D Receptor Gene TaqI, BsmI and FokI Polymorphisms in Korean Patients with Tuberculosis

• Kang TJ
• Jin SH
• Yeum CE
• Lee SB
• Kim CH
• Lee SH
• Kim KH
• Shin ES
• Chae GT

• KMID: 2167997
• DOI: http://doi.org/10.4110/in.2011.11.5.253

Abstract

BACKGROUND
The active metabolite (1, 25-dihydroxycholecalciferol) of vitamin D (25-hydroxycholecalciferol) leads to activation of macrophages and deficiency of vitamin D seems to be involved in the risk of tuberculosis. The effects of vitamin D are exerted by interaction with the vitamin D receptor (VDR) and may be influenced by polymorphism in the VDR gene. In this study, variation in the VDR gene was investigated in Korean population with tuberculosis.
METHODS
We typed three VDR polymorphisms of restriction endonuclease sites for TaqI, BsmI and FokI in 155 patients with tuberculosis and 105 healthy volunteers.
RESULTS
The frequencies of FokI genotypes determined from TB patients were 29.13% for FF, 56.31% for Ff, and 14.56% for ff. We observed 1.4-fold increased prevalence of the Ff genotype in TB patients compared with normal healthy groups (p=0.0857). However, there was no significant association between the genotype groups, TB patient and normal control, for FokI polymorphism. There was also no significant association between VDR gene and tuberculosis in another polymorphism (BsmI and TaqI).
CONCLUSION
Three polymorphisms (TaqI, BsmI and FokI) in the VDR gene do not appear to be responsible for host susceptibility to human tuberculosis in Korean population.

Keyword

VDR; Polymorphism; Tuberculosis; Korean; FokI

MeSH Terms

DNA Restriction Enzymes
Genotype
Humans
Macrophages
Prevalence
Receptors, Calcitriol
Tuberculosis
Vitamin D
Vitamins
DNA Restriction Enzymes
Receptors, Calcitriol
Vitamin D
Vitamins

Figure

• 

  Figure 1 The human vitamin D recepter (VDR) chromosomal genes, containing a total 11 exons, are variably present in VDR transcripts. Three polymorphisms, including a FokI, BsmI, and TaqI site, are shown and discussed in the text. The figure is adapted from the schematic view in reference 4.

• 

  Figure 2 FokI restriction patterns of the various genotypes. PCR products before (A) and after (B) treatment of enzyme were showed. B. Three restriction fragments were present at 266, 193, and 73 bp. The FF genotype (B-1) had only the 266 bp band, the ff genotype (B-3) had the two bands at 193 and 73 bp, and the Ff heterozygous genotype (B-2) had the three bands.

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