J Korean Acad Nurs.  2015 Jun;45(3):367-377. 10.4040/jkan.2015.45.3.367.

Effects of Blending Oil of Lavender and Thyme on Oxidative Stress, Immunity, and Skin Condition in Atopic Dermatitis Induced Mice

• Seo YM
• Jeong SH

• KMID: 2164378
• DOI: http://doi.org/10.4040/jkan.2015.45.3.367

Abstract

PURPOSE
The purpose of this study was to evaluate the effects of essential oil on oxidative stress, immunity, and skin condition in atopic dermatitis (AD) induced mice.
METHODS
This study was a 3x3 factorial design. Factors were oil type (Lavender, Thyme, and 2:1 mixture of lavender and thyme oil [blending oil]) and treatment period (0 day, 7 days, and 21 days). The samples were 45 mice with AD and randomly assigned to nine groups of five mice per group. The dependent variables such as superoxide radical, IgE, degranulated mast cells, and epidermal thickness were measured. Data were collected from February to April in 2014. Descriptive statistics, One-way ANOVA, Two-way ANOVA, and Tukey's HSD test were performed using the SPSS WIN 20.0 program.
RESULTS
Dependent variables were not statistically significantly different by the three oil types (p >.05). Essential oils such as lavender, thyme, and blending oil were all effective in reducing AD symptoms and especially 2:1 blending oil were most effective. There were statistically significant differences by the three treatment periods in all dependent variables (p <.001). There were statistically significant interactions between oil types and treatment periods in all dependent variables (p <.01). For decreasing superoxide radical, degranulated mast cells, and epidermal thickness, 2:1 mixed oil should be applied for at least 21 days. Otherwise to reduce IgE, 2:1 mixed oil should be used for at least 7 days.
CONCLUSION
These findings provide bases for developing effective interventions for AD patients to manage their AD symptoms.

Keyword

Atopic dermatitis; Essential oil; Superoxide radical; IgE; Mast cell

MeSH Terms

Animals
Dermatitis, Atopic/chemically induced/*drug therapy/pathology
Disease Models, Animal
*Immunity/drug effects
Immunoglobulin E/blood
Lavandula/*chemistry/metabolism
Mast Cells/cytology/metabolism
Mice
Oils, Volatile/chemistry/pharmacology/therapeutic use
*Oxidative Stress/drug effects
Picryl Chloride/toxicity
Plant Oils/chemistry/pharmacology/*therapeutic use
Singlet Oxygen/metabolism
Skin/drug effects/pathology
Thymus Plant/*chemistry/metabolism
Immunoglobulin E
Oils, Volatile
Picryl Chloride
Plant Oils
Singlet Oxygen

Figure

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  Figure 1 Measured variable on skin barrier.

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  Figure 2 Photographs of skin in atopic dermatitis induced mice (1 to 9 number means the treatment period and oil type).

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