Korean J Blood Transfus.  2016 Apr;27(1):55-67. 10.17945/kjbt.2016.27.1.55.

In vitro Quality Evaluation of Apheresis Platelets in Four Kinds of Platelet Additive Solutions

• Kim J
• Kang J
• Kwon SY
• Oh DJ

• KMID: 2163121
• DOI: http://doi.org/10.17945/kjbt.2016.27.1.55

Abstract

BACKGROUND
Platelets (PLTs) stored in platelet additive solution (PAS) presents potential benefits in clinical use by reducing the risk of several plasma-associated adverse transfusion reactions and more plasma may be recovered for fractionation. In this study, we compared in vitro characteristics of apheresis PLTs stored in CompoSol PS (Fenwal, Lake Zurich, IL, USA), InterSol (Fenwal, Lake Zurich, IL, USA), SSP+ (MacoPharma, Tourcoing, France), T-PAS+ (Terumo BCT, Lakewood, CO, USA), or plasma to evaluate the effectiveness of PAS.
METHODS
PLTs were collected two times by apheresis from 12 healthy volunteers in a study comparing four kinds of PASs with 35% autologous plasma and 100% plasma-stored apheresis PLTs. The parameters of PLTs, including PLT counts, pH, PLT activation markers, blood gases, and metabolic variables were assessed up to 7-day.
RESULTS
The results of in vitro assay including PLT concentration, mean PLT volume, pH, and blood gases for PLTs in four kinds of PASs were similar to those in 100% plasma PLTs. All units had Day 5 pH greater than 6.2. In vitro quality rating results, PLTs in T-PAS+ had a rating of 5, 4 for CompoSol PS, 2 for SSP+, 1 for InterSol, and 2 for plasma on Day 5.
CONCLUSION
Partial replacement of plasma with CompoSol PS, SSP+, or T-PAS+ in PLTs showed better or equivalent quality and preservability of PLTs compared to PLTs in 100% plasma. The use of PAS for storage of PLTs in clinical practice may have an advantage as PAS-stored PLTs have a reduced volume of plasma.

Keyword

Apheresis platelets; Platelets additive solution; Storage

MeSH Terms

Blood Component Removal*
Blood Group Incompatibility
Blood Platelets*
Gases
Healthy Volunteers
Hydrogen-Ion Concentration
Lakes
Plasma
Gases