Ann Dermatol.  2011 May;23(2):156-161. 10.5021/ad.2011.23.2.156.

Distribution of Malassezia Species on the Scalp in Korean Seborrheic Dermatitis Patients

• Lee YW
• Byun HJ
• Kim BJ
• Kim DH
• Lim YY
• Lee JW
• Kim MN
• Kim D
• Chun YJ
• Mun SK
• Kim CW
• Kim SE
• Hwang JS

• KMID: 2156656
• DOI: http://doi.org/10.5021/ad.2011.23.2.156

Abstract

BACKGROUND
Malassezia species play an important role in the pathogenesis of seborrheic dermatitis. In particular, M. restricta and M. globosa are considered to be the predominant organisms in seborrheic dermatitis of Western countries. However, species distribution of Malassezia in seborrheic dermatitis has not been clearly determined yet in Asia.
OBJECTIVE
To identify the distribution of Malassezia species on the scalp of seborrheic dermatitis patients in Korea using 26S rDNA PCR-RFLP analysis.
METHODS
A total of 40 seborrheic dermatitis patients and 100 normal healthy volunteers were included in this study. For the identification of Malassezia species, the scalp scales of the subjects were analyzed by 26S rDNA PCR-RFLP analysis.
RESULTS
The most commonly identified Malassezia species were M. restricta in the seborrheic dermatitis patients, and M. globosa in the normal controls. In the seborrheic dermatitis group, M. restricta was identified in 47.5%, M. globosa in 27.5%, M. furfur in 7.5%, and M. sympodialis in 2.5% of patients. In the healthy control group, M. globosa was identified in 32.0%, M. restricta in 25.0%, M. furfur in 8.0%, M. obtusa in 6.0%, M. slooffiae in 6.0%, and M. sympodialis in 4.0% of subjects.
CONCLUSION
M. restricta is considered to be the most important Malassezia species in Korean seborrheic dermatitis patients.

Keyword

26S rDNA PCR-RFLP; Malassezia; Seborrheic dermatitis

MeSH Terms

Dermatitis, Seborrheic
DNA, Ribosomal
Humans
Korea
Malassezia
Scalp
Weights and Measures
DNA, Ribosomal

Figure

• 

  Fig. 1 26S rDNA PCR in the six Malassezia standard strains. The size of PCR products were almost 580 bp. Lane M: marker, lane 1: M. furfur, lane 2: M. globosa, lane 3: M. restricta, lane 4: M. obtusa, lane 5: M. sympodialis, lane 6: M. slooffiae.

• 

  Fig. 2 26S rDNA PCR-RFLP pattern in the six Malassezia standard strains. 26S rDNA PCR products were digested with two restriction enzymes. (A) Hha1; all the six standard strains showed different RFLP patterns. (B) BstF51; M. globosa and M. obtusa showed the same RFLP patterns. The other four strains all showed different patterns. Lane M: marker, lane 1: M. furfur, lane 2: M. globosa, lane 3: M. restricta, lane 4: M. obtusa, lane 5: M. sympodialis, lane 6: M. slooffiae.

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