J Korean Neurosurg Soc.  2016 Jan;59(1):26-36. 10.3340/jkns.2016.59.1.26.

Prognostic Role of Methylation Status of the MGMT Promoter Determined Quantitatively by Pyrosequencing in Glioblastoma Patients

Affiliations
  • 1Department of Pathology, Dong-A University College of Medicine, Busan, Korea.
  • 2Department of Neurosurgery, Dong-A University College of Medicine, Busan, Korea.
  • 3Division of Neurooncology, Department of Neurosurgery, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon, Korea. yzkim@skku.edu

Abstract


OBJECTIVE
This study investigated whether pyrosequencing can be used to determine the methylation status of the MGMT promoter as a clinical biomarker using relatively old archival tissue samples of glioblastoma. We also examined other prognostic factors for survival of glioblastoma patients.
METHODS
The available study set included formalin-fixed paraffin-embedded (FFPE) tissue from 104 patients at two institutes from 1997 to 2012, all of which were diagnosed histopathologically as glioblastoma. Clinicopathologic data were collected by review of medical records. For pyrosequencing analysis, the PyroMark Q96 CpG MGMT kit (Qiagen, Hilden, Germany) was used to detect the level of methylation at exon 1 positions 17-39 of the MGMT gene, which contains 5 CpGs.
RESULTS
Methylation of the MGMT promoter was detected in 43 (41.3%) of 104 samples. The average percentage methylation was 14.0+/-16.8% overall and 39.0+/-14.7% for methylated cases. There was no significant pattern of linear increase or decrease according to the age of the FFPE block (p=0.687). In multivariate analysis, age, performance status, extent of surgery, method of adjuvant therapy, and methylation status estimated by pyrosequencing were independently associated with overall survival. Additionally, patients with a high level of methylation survived longer than those with low methylation (p=0.016).
CONCLUSION
In this study, the status and extent of methylation of the MGMT promoter analyzed by pyrosequencing were associated with overall survival in glioblastoma patients. Pyrosequencing is a quantitative method that overcomes the problems of MSP and a simple technique for accurate analysis of DNA sequences.

Keyword

MGMT promoter; Methylation; Glioblastoma; Prognosis; Pyrosequencing

MeSH Terms

Academies and Institutes
Base Sequence
Exons
Glioblastoma*
Humans
Medical Records
Methylation*
Multivariate Analysis
Prognosis

Figure

  • Fig. 1 Two representative glioblastoma pyrograms. Five consecutive positions are analyzed for CpGs; the last one is an internal control for bisulfite treatment. Each C peak in a yellow background represents the methylation percentage for each CpG. A : Unmethylated MGMT promoter of a glioblastoma sample. B : Methylated MGMT promoter of a glioblastoma sample.

  • Fig. 2 Kaplan-Meier survival curves for patients with glioblastoma. A : Age <50 vs. age ≥50. B : KPS ≥70 vs. KPS <70. C : Extent of surgical resection. D : Method of adjuvant treatment. E : Methylation status of the MGMT promoter analyzed by methylation-specific PCR. F : Methylation status of the MGMT promoter analyzed by pyrosequencing. G : Methylation status of MGMT promoter in patients who were treated with concurrent temozolomide chemoradiotherapy (TMZ CCRT). H : Extent of methylation of the MGMT promoter analyzed quantitatively by pyrosequencing.


Reference

1. Derks S, Lentjes MH, Hellebrekers DM, de Bruïne AP, Herman JG, van Engeland M. Methylation-specific PCR unraveled. Cell Oncol. 2004; 26:291–299. PMID: 15623939.
Article
2. Dunn J, Baborie A, Alam F, Joyce K, Moxham M, Sibson R, et al. Extent of MGMT promoter methylation correlates with outcome in glioblastomas given temozolomide and radiotherapy. Br J Cancer. 2009; 101:124–131. PMID: 19536096.
Article
3. Eng J. Receiver operating characteristic analysis : a primer. Acad Radiol. 2005; 12:909–916. PMID: 16039544.
4. Esteller M, Garcia-Foncillas J, Andion E, Goodman SN, Hidalgo OF, Vanaclocha V, et al. Inactivation of the DNA-repair gene MGMT and the clinical response of gliomas to alkylating agents. N Engl J Med. 2000; 343:1350–1354. PMID: 11070098.
Article
5. Etcheverry A, Aubry M, de Tayrac M, Vauleon E, Boniface R, Guenot F, et al. DNA methylation in glioblastoma : impact on gene expression and clinical outcome. BMC Genomics. 2010; 11:701. PMID: 21156036.
6. Frommer M, McDonald LE, Millar DS, Collis CM, Watt F, Grigg GW, et al. A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands. Proc Natl Acad Sci U S A. 1992; 89:1827–1831. PMID: 1542678.
Article
7. Hegi ME, Diserens AC, Gorlia T, Hamou MF, de Tribolet N, Weller M, et al. MGMT gene silencing and benefit from temozolomide in glioblastoma. N Engl J Med. 2005; 352:997–1003. PMID: 15758010.
Article
8. Herman JG, Graff JR, Myöhänen S, Nelkin BD, Baylin SB. Methylation-specific PCR : a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci U S A. 1996; 93:9821–9826. PMID: 8790415.
Article
9. Jung KW, Ha J, Lee SH, Won YJ, Yoo H. An updated nationwide epidemiology of primary brain tumors in republic of Korea. Brain Tumor Res Treat. 2013; 1:16–23. PMID: 24904884.
Article
10. Karayan-Tapon L, Quillien V, Guilhot J, Wager M, Fromont G, Saikali S, et al. Prognostic value of O6-methylguanine-DNA methyltransferase status in glioblastoma patients, assessed by five different methods. J Neurooncol. 2010; 97:311–322. PMID: 19841865.
Article
11. Lantos PL, Louis DN, Rosenblum Mk, Kleihues P. Tumors of the Nervous System. London: Oxford University Press;2002.
12. Lee SH, Hwang TS, Koh YC, Kim WY, Han HS, Kim WS, et al. A consideration of MGMT gene promotor methylation analysis for glioblastoma using methylation-specific polymerase chain reaction and pyrosequencing. Korean J Pathol. 2011; 45:21–29.
Article
13. Marsh S. Pyrosequencing applications. Methods Mol Biol. 2007; 373:15–24. PMID: 17185754.
14. Mikeska T, Bock C, El-Maarri O, Hübner A, Ehrentraut D, Schramm J, et al. Optimization of quantitative MGMT promoter methylation analysis using pyrosequencing and combined bisulfite restriction analysis. J Mol Diagn. 2007; 9:368–381. PMID: 17591937.
Article
15. Park JW, Hwang JH. The long-term survival in patients with anaplastic astrocytoma or glioblastoma. J Korean Neurosurg Soc. 2003; 34:514–520.
16. Palmisano WA, Divine KK, Saccomanno G, Gilliland FD, Baylin SB, Herman JG, et al. Predicting lung cancer by detecting aberrant promoter methylation in sputum. Cancer Res. 2000; 60:5954–5958. PMID: 11085511.
17. Provenzale JM, Ison C, Delong D. Bidimensional measurements in brain tumors : assessment of interobserver variability. AJR Am J Roentgenol. 2009; 193:W515–W522. PMID: 19933626.
18. Singer-Sam J, Grant M, LeBon JM, Okuyama K, Chapman V, Monk M, et al. Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation. Mol Cell Biol. 1990; 10:4987–4989. PMID: 1697035.
Article
19. Stupp R, Hegi ME, Mason WP, van den Bent MJ, Taphoorn MJ, Janzer RC, et al. Effects of radiotherapy with concomitant and adjuvant temozolomide versus radiotherapy alone on survival in glioblastoma in a randomised phase III study : 5-year analysis of the EORTC-NCIC trial. Lancet Oncol. 2009; 10:459–466. PMID: 19269895.
Article
20. Verbeek B, Southgate TD, Gilham DE, Margison GP. O6-Methylguanine-DNA methyltransferase inactivation and chemotherapy. Br Med Bull. 2008; 85:17–33. PMID: 18245773.
Article
21. Vlassenbroeck I, Califice S, Diserens AC, Migliavacca E, Straub J, Di Stefano I, et al. Validation of real-time methylation-specific PCR to determine O6-methylguanine-DNA methyltransferase gene promoter methylation in glioma. J Mol Diagn. 2008; 10:332–337. PMID: 18556773.
Article
22. Watts GS, Pieper RO, Costello JF, Peng YM, Dalton WS, Futscher BW. Methylation of discrete regions of the O6-methylguanine DNA methyltransferase (MGMT) CpG island is associated with heterochromatinization of the MGMT transcription start site and silencing of the gene. Mol Cell Biol. 1997; 17:5612–5619. PMID: 9271436.
Article
Full Text Links
  • JKNS
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr