J Korean Med Sci.  2010 Jun;25(6):863-867. 10.3346/jkms.2010.25.6.863.

The Relationship between the Presence of Chromosomal Instability and Prognosis of Squamous Cell Carcinoma of the Lung: Fluorescence in situ Hybridization Analysis of Paraffin-embedded Tissue from 47 Korean Patients

• Yoo JW
• Seo KW
• Jang SJ
• Oh YM
• Shim TS
• Kim WS
• Lee DS
• Lee SD
• Choi CM

• KMID: 2150864
• DOI: http://doi.org/10.3346/jkms.2010.25.6.863

Abstract

To evaluate the prognostic importance of chromosomal instability (CIN) in squamous cell carcinoma (SCC) of the lung, the relationship between CIN detected by fluorescence in situ hybridization (FISH) and survival in SCC patients was examined. Forty-seven surgical specimens of lung SCC were analyzed. To identify tumors with CIN, p16 and multi-target DNA FISH assays for c-myc, chromosome 6, EGFR, and chromosome 5 (LAVysion, Vysis) were performed on nuclei extracted from paraffin-embedded tumor tissues. Survival rates were compared in terms of age, T factor, N factor, CIN, and smoking status. A sample was defined as CIN-positive if at least four of the five chromosomes were positive. Among the 47 specimens, 9 (19%) were CIN-positive. The overall survival rate was 66%. Overall survival rates were estimated as 33.3% for CIN-positive patients and 76.7% for CIN-negative patients (Hazard ratio 3.47; 95% Confidence interval, 1.25-9.67; P=0.017). In multivariate analysis, the presence of CIN was a predictive factor for survival. CIN-positive based on FISH can be prognostic factor of lung SCC.

Keyword

Chromosomal Instability; In Situ Hybridization, Fluorescence; Lung Neoplasms; Carcinoma, Squamous Cell

Figure

• 

  Fig. 1 Image of fluorescent in situ hybridization (FISH) performed on extracted nuclei of lung cancer tissue. (A) LaVysion FISH: cell showing increase copy number of 7p12, chromosome 5 and 8q24 region. A Cell shows five red signals (7p12 region), three Gold signals (8q 24 region), four green signals (5p15.2) and two aqua signals (chromosome 6). (B) p16 FISH: cell with deletion p 16 gene. A cell shows homozygous deletion of p16 gene (no orange signal) on normal number of chromosome 9 (two green signal).

• 

  Fig. 2 Overall survival curve according to CIN status (P=0.013).

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