Immune Netw.  2005 Jun;5(2):99-104. 10.4110/in.2005.5.2.99.

A Combination of PG490 and Lipopolysaccharide Induce Apoptosis through Activation of Casapase-3 and Down- regulation of cIAP1 and XIAP in Human Astroglioma Cell

• Lee TJ
• Woo KJ
• Park JW
• Kwon TK

• KMID: 2150603
• DOI: http://doi.org/10.4110/in.2005.5.2.99

Abstract

BACKGROUND
Malignant gliomas are the most common primary tumors in the central nervous system. METHODS: We investigated the combined effect of PG490 and LPS on the induction of the apoptotic pathway in human astroglioma cells. RESULTS: Treatment of U87 cells with combination of 50 nM of PG490 and 50microgram/ml of LPS resulted in increased internucleosomal DNA fragmentation, cleavage of PLC-gamma1, and down- regulation of cIAP1 and XIAP. The combination of LPS and PG490 treatment-induced apoptosis is mediated through the activation of caspase, which is inhibited by the caspase inhibitor, z-VAD-fmk. Also, release of cytochrome c was found in PG490 and LPS- cotreated U87 cell. CONCLUSION: Taken together, combination of PG490 and LPS appears to be a potent inducer of apoptosis in astrogliaoma cells, and might have some benefit in the treatment of glioma patients.

Keyword

LPS; PG490; apoptosis; astroglioma

MeSH Terms

Apoptosis*
Astrocytoma*
Central Nervous System
Cytochromes c
DNA Fragmentation
Glioma
Humans*
Cytochromes c