Restor Dent Endod.  2015 May;40(2):143-148. 10.5395/rde.2015.40.2.143.

Effect of three different irrigation solutions applied by passive ultrasonic irrigation

• Llena C
• Forner L
• Cambralla
• Lozano A

• KMID: 2148864
• DOI: http://doi.org/10.5395/rde.2015.40.2.143

Abstract

OBJECTIVES
This study evaluated the maximum depth and percentage of irrigant penetration into dentinal tubules by passive ultrasonic irrigation (PUI).
MATERIALS AND METHODS
Thirty extracted human teeth were instrumented and divided into three groups. According to final irrigation regimen, 5.25% sodium hypochlorite (Group A, NaOCl), 2% chlorhexidine (Group B, CHX) and saline solution (Group C, control group) were applied with Irrisafe 20 tips (Acteon) and PUI. Irrigant was mixed with 0.1% rhodamine B. Sections at 2 mm, 5 mm, and 8 mm from the apex were examined with confocal laser scanning microscopy (CLSM). The percentage and maximum depth of irrigant penetration were measured. Kruskal-Wallis test and Mann-Whitney test were performed for overall comparison between groups at each level and for pairwise comparison, respectively. Within a group, Wilcoxon test was performed among different levels. p values less than 0.05 were considered significant.
RESULTS
In all groups, highest penetration depth and percentage of penetration were observed at the 8 mm level. At 2 mm level, Groups A and B had significantly greater depths and percentages in penetration than Group C (p < 0.05), but there were no significant differences between Groups A and B. At 5 mm level, penetration depths and percentage of penetration was not significantly different among the groups.
CONCLUSIONS
NaOCl and CHX applied by PUI showed similar depth and percentage of penetration at all evaluated levels.

Keyword

Chlorhexidine; Confocal laser scanning microscope; Dentinal penetration; Passive ultrasonic irrigation; Sodium hypochlorite

MeSH Terms

Chlorhexidine
Dentin
Humans
Microscopy, Confocal
Rhodamines
Sodium Chloride
Sodium Hypochlorite
Tooth
Ultrasonics*
Chlorhexidine
Rhodamines
Sodium Chloride
Sodium Hypochlorite

Figure

• 

  Figure 1 Representative confocal laser scanning microscopic images. Only stained irrigant can be seen with this technique. (a) The image shows fluorescence around the canal walls without penetration into dentinal tubules; (b) Deep penetration is shown in part of the canal wall. The green line was used to mark and to measure the percentage of canal wall showing dentinal tubule penetration; (c) An intense irrigant-labelled penetration is shown. The maximum penetration into dentinal tubules was measured in each case (green line).

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