Korean J Clin Microbiol.
2006 Apr;9(1):24-29.
Molecular Epidemiologic Analysis of Legionella pneumophila by Infrequent-Restriction-Site Polymerase Chain Reaction
- Affiliations
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- 1Department of Laboratory Medicine, Dong-A University College of Medicine, Korea.
- 2Department of Laboratory Medicine, Pusan National University College of Medicine, Korea. CCHL@pusan.ac.kr
- 3Microbiology Division, Busan Metropolitan City Institute of Health and Environment, Busan, Korea.
Abstract
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BACKGROUND: The frequent outbreak of legionellosis makes it critical to identify infection sources for the prevention and blockade of transmission of the disease.
METHODS
Thirty-one strains of Legionella pneumophila isolated from the cooling towers of big buildings in Busan and Gyungsangnamdo Province areas, 12 strains of L. pneumophila from patients in Japan, and one type strain (L. pneumophila ATCC 33152) were used for molecular strain typing by using an infrequent-restriction-site polymerase chain reaction (IRS-PCR).
RESULTS
Each strain revealed to have 7-16 bands of 200-1000 bp size. All 44 strains showed band patterns different from each other, except two strains sharing 90% homology.
CONCLUSION
The molecular typing of Legionellaby IRS-PCR is an excellent and rapid method for discriminating strains; therefore, it should be useful in demonstrating the identity of possible outbreak strains.
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