J Korean Soc Microbiol.
1998 Aug;33(4):353-360.
Immunogenecity of Outer Membrane Proteins of vibro vulnificus
- Affiliations
-
- 1Department of Microbiology, College of Natural Science, Pusan National University, Pusan, Korea. crjung@hyowon.pusan.ac.kr
- 2Department of Microbiology, Graduate School Pusan National University, Pusan, Korea.
- 3Biochemical Research Team, Cheil Food and Chemical INC. Kyonggi-do, Korea.
Abstract
- To provide basic information on the pathogengenesis of Vibrio vulnificus infection and for the manufacture of effective vaccine, outer membrane proteins (OMPs) were extracted from V. vulnificus ATCC 27562 strain and analysed by 12% of sodium dodecyl sulfate-polyarylamide gel(SDS-PAGE). Major 36 kDa and 48 kDa, 46 kDa, 66 kDa and 24 kDa protein bands appeared on gel by Coomassie stain and determined by densitometer. Immunogenecity of these proteins was examined by enzyme-linked immunosorbant assay(ELISA) and western blotting with rabbit anti-V. vulnificus serum. OMPs reacted with this antiserum, and the major 36 kDa protein appeared most immunogenic.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Isolation and identification of Vibrio vulnificus and Vibrio parahaemolyticus from coast of Pusan and Daechon
- Isolation and Detection of Vibrio vulnificus from the Southern Sea of Korea for the Prevention of V. vulnificus Infection (The Characteristics of Vibrio vulnificus Isolates from the Southern Sea of Korea)
- The Attachment of Detergent-Extracted Outer Membrane Proteins of Orientia tsutsugamushi to the Host Cell Surface
- The Effect of Iron Limmted Condition on Outer Membrane of Vibrio mimicus
- Surface interactions between two of the main periodontal pathogens: Porphyromonas gingivalis and Tannerella forsythia
