J Korean Soc Microbiol.  1998 Aug;33(4):353-360.

Immunogenecity of Outer Membrane Proteins of vibro vulnificus

Affiliations
  • 1Department of Microbiology, College of Natural Science, Pusan National University, Pusan, Korea. crjung@hyowon.pusan.ac.kr
  • 2Department of Microbiology, Graduate School Pusan National University, Pusan, Korea.
  • 3Biochemical Research Team, Cheil Food and Chemical INC. Kyonggi-do, Korea.

Abstract

To provide basic information on the pathogengenesis of Vibrio vulnificus infection and for the manufacture of effective vaccine, outer membrane proteins (OMPs) were extracted from V. vulnificus ATCC 27562 strain and analysed by 12% of sodium dodecyl sulfate-polyarylamide gel(SDS-PAGE). Major 36 kDa and 48 kDa, 46 kDa, 66 kDa and 24 kDa protein bands appeared on gel by Coomassie stain and determined by densitometer. Immunogenecity of these proteins was examined by enzyme-linked immunosorbant assay(ELISA) and western blotting with rabbit anti-V. vulnificus serum. OMPs reacted with this antiserum, and the major 36 kDa protein appeared most immunogenic.

Keyword

Vibrio vulnificus; Outer membrane protein; ELISA; Western blotting

MeSH Terms

Blotting, Western
Enzyme-Linked Immunosorbent Assay
Membrane Proteins*
Membranes*
Sodium
Vibrio vulnificus
Membrane Proteins
Sodium
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