Korean J Blood Transfus.  2015 Dec;26(3):249-256. 10.17945/kjbt.2015.26.3.249.

Analysis of Unexpected Antibodies Detected in Children: A Single Center Study for 7 Years

• Lee HJ
• Jo SY
• Shin KH
• Song D
• Lee SM
• Kim IS
• Chang CL
• Kim HH

• KMID: 2139789
• DOI: http://doi.org/10.17945/kjbt.2015.26.3.249

Abstract

BACKGROUND
Frequency and distribution of unexpected red cell antibodies vary according to study population and method of antibody detection. The frequency and specificities of unexpected red cell antibodies were analyzed and compared in adults and children.
METHODS
We analyzed the results of antibody screening and identification tests performed from November of 2008 to June of 2015 at Pusan National University Yangsan Hospital. A commercially available three-cell antigen panel, DiaCell I, II and Dia (DiaMed, Murten, Switzerland) for antibody screening, LISS/Coombs and NaCl/Enzyme card and the ID-Dia Panel (DiaMed, Murten, Switzerland) for antibody identification were used.
RESULTS
Among the 91,145 adults, 1,804 (1.97%) had positive results for antibody screening test and 11,457 children, 447 (3.90%) had positive results. In adults, the most frequently detected unexpected antibody was anti-E (103 samples), followed by by anti-E&c (51 samples), anti-Lea (27 samples), and anti-Dia (27 samples). In children, anti-M (7 samples), anti-E&c (5 samples), anti-E (4 samples), and anti-D (4 samples) were most frequently detected. Among 9 pediatric patients with anti-E or anti-E&c, 5 patients were proven as hemolytic disease of the fetus and newborn.
CONCLUSION
In this study, the positive rate of unexpected antibody screening in children was higher than in adults. Distribution of unexpected antibody differed between children and adults. It may be related to the frequent cardiac surgery performed in children at our hospital. No studies have been conducted in children. Our research may provide data for the frequency and characteristics of red cell antibodies in children.

Keyword

Unexpected antibody; Frequency; Children

MeSH Terms

Adult
Antibodies*
Busan
Child*
Fetus
Gyeongsangnam-do
Humans
Infant, Newborn
Mass Screening
Thoracic Surgery
Antibodies