Korean J Blood Transfus.  2009 Apr;20(1):23-31.

The Frequency and Distribution of Unexpected Antibodies in Transfusion Candidates with the Use of the Ortho BioVue System: Recent Four Year Experience

Affiliations
  • 1Department of Laboratory Medicine, Kyung Hee University College of Medicine, Seoul, Korea. peanut500@hanmail.net

Abstract

BACKGROUND
Unexpected antibodies are important factors for hemolytic transfusion reactions. In the past, the tube method was used for detecting unexpected antibodies. The column agglutination method has recently been widely used because of its simplicity and it has a higher rate of detecting warm antibodies. In this study, we describe the frequency and distribution of unexpected antibodies in transfusion candidates during the recent 4 years and the transfusion characteristics in the identified cases.
METHODS
Antibody screening tests were carried out on 44,008 sera using the column agglutination method from January, 2005 to December, 2008. The antibodies were screened and identified by the Ortho BioVue System (Ortho-Clinical Diagnostics, Raritan, NJ, USA).
RESULTS
Of the 44,008 cases that underwent unexpected antibodies screening, 589 cases (1.3%) showed positive results. Unexpected antibodies were identified in 383 cases. The antibodies that were most frequently detected were anti-Lewis antibodies in 130 cases (34.0%). Among the warm antibodies, anti-Rh and anti-Kidd antibodies were detected in 67 cases (17.5%) and 2 cases (0.5%), respectively. Unidentified antibodies were detected in 133 cases (38.9%). Among the patients with unexpected antibodies, 137 cases (35.8%) had a history of previous transfusion and 244 cases (63.7%) had a history of previous transfusion or gestation.
CONCLUSION
Anti-Lewis cold antibodies were the most frequently detected antibodies. Warm antibodies were also frequently detected, and these are clinically significant.

Keyword

Unexpected antibodies; Column agglutination method; Antibody screening and identification test

MeSH Terms

Agglutination
Antibodies
Blood Group Incompatibility
Cold Temperature
Humans
Mass Screening
Antibodies
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