Nutr Res Pract.  2008 Dec;2(4):234-239. 10.4162/nrp.2008.2.4.234.

Pre- and post-initiation modulating effects of green tea ingestion on rat hepatocarcinogenesis

• Kim HS
• Kim HS
• Choi H

• KMID: 2139197
• DOI: http://doi.org/10.4162/nrp.2008.2.4.234

Abstract

The purpose of this study was to investigate the effects of green tea ingestion on hepatocarcinogenesis before and after its initiation. Male Sprague-Dawley rats were fed an AIN76A diet with or without green tea. Initiation was induced by a single dose (200 mg/kg) of diethylnitrosamine at week 4 and 0.02% (w/w) 2-acetylaminofluorene was supplied in the diets. The control group had free access to water for 13 weeks (CTR13). Tea infusion was provided from the beginning of the experiment for 13 weeks (PRE13) or from the post-initiation stage until week 13 (POST13). Three other groups (CTR24, PRE24 and POST24) were added to examine the longer-term effects (24 weeks) with the same experimental design. The percentage area of liver sections that were positive for hepatic placental glutathione S-transferase (GST-P), which was used as a marker of preneoplastic lesions, was smaller in PRE13 (20.2 +/- 5.0%, mean +/- SD) and POST13 (26.0 +/- 4.8%) than in CTR13 (33.2 +/- 5.8%, p<0.05). Over the longer period, the GST-P lesions were significantly smaller for both PRE24 and POST24 (21.6 +/- 8.5% and 22.2 +/- 4.0%, respectively) than for CTR24 (28.6 +/- 5.1%, p<0.05), but there was no significant difference between PRE24 and POST24. The liver content of thiobarbituric acid reactive substances was significantly lower in the tea groups than in the controls (p<0.05). However, no significant differences were observed among groups of GST activity. The results show that tea consumption exhibits a stronger short-term initiation-inhibiting ability in liver carcinogenesis, but over a longer period, the preventive effects of green tea ingestion do not differ in post- and pre-initiation.

Keyword

Green tea; hepatocarcinogenesis; initiation; GST-P; rats

MeSH Terms

2-Acetylaminofluorene
Animals
Diet
Diethylnitrosamine
Eating
Glutathione Transferase
Humans
Liver
Male
Rats
Rats, Sprague-Dawley
Research Design
Tea
Thiobarbiturates
Thiobarbituric Acid Reactive Substances
Water
2-Acetylaminofluorene
Diethylnitrosamine
Glutathione Transferase
Tea
Thiobarbiturates
Thiobarbituric Acid Reactive Substances
Water

Figure

• 

  Fig. 1 Experimental design. ↑, 200mg DEN in 2.0ml saline/kg BW i.p. injection; □, AIN 76A diet and deionized water; ▒, AIN 76A diet and green tea as a sole drinking solution; ░, AIN 76A with 0.02% AAF in diet; ■, AIN 76A with 0.02% AAF in diet and green tea as a sole drinking solution; †, sacrifice. CTR13, control group for 13 wk; POST13, green tea ingestion for 7 wk from the post-initiation period; PRE13, green tea ingestion fore 13 wk, preinitiation; PRE24, green tea ingestion for 24 wk, preinitiation; POST24, green tea ingestion for 18 wk, post-initiation period; CTR24, control group for 24 wk.

• 

  Fig. 2 Effect of green tea ingestion on the area of placental glutathione S-transferase positive foci. Means without a common letter differ (p<0.05) with ANOVA and Duncan's multiple post-hoc test.

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