J Korean Assoc Maxillofac Plast Reconstr Surg.  2011 Nov;33(6):467-477.

The Effect of Low-intensity Pulsed Ultrasound on Osteogenesis in Mini-pig Mandibles

• Yun YE
• Lee J
• Min SK
• Kim SJ

Abstract

PURPOSE
The objective of this study was to examine the affected period and the amount of bone formation during osteogenesis of intramembranous bone using low-intensity pulsed ultrasound (LPUS) in vivo.
METHODS
Xeno-bone (Bio-oss) and autogenous bone were grafted bilaterally into mini-pig mandibles. The left mandible served as the control and the other mandible was treated with 3 MHz, 160 mW (output, 0.8 mW) ultrasound stimulation for 7 days 15 minutes per day. The mini-pigs were sacrificed at 1, 2, 4, and 8 weeks, and micro computed tomography (microCT), a microscopic examination, and a statistical analysis were performed on the specimens.
RESULTS
Based on a computerized image analysis of the microCT scans, the experimental group had an average 150% more new bone formation than that in the control group. The effect of LPUS continued during the post operative 2 weeks. The histomorphological microscopic examination showed similar results.
CONCLUSION
Our results suggest the LPUS had an effect on early intramembranous bone formation in vivo.

Keyword

Low-intensity pulsed ultrasound; in vivo; Intramembranous bone; microCT

MeSH Terms

Mandible
Osteogenesis
Transplants
X-Ray Microtomography