J Clin Pathol Qual Control.  1998 Jun;20(1):193-198.

Blood Culture Experience using BACTEC 9240

• Kim HS
• Cho DH

Abstract

BACKGROUND: Blood culture is an important procedure for the determination of the etiologic agent of bacteremia. Rapid and accurate blood culture results can provide clinicians with very important informations for the diagnosis and appropriate treatment of the patient.
METHODS
Isolates taken from blood culture using BACTEC 9240 from January to December in 1997 were analyzed and compared to 1996's results. Organism identifications were based on conventional method or commercial kit system.
RESULTS
In 1996, a total of 3,027 blood specimens were cultured by conventional manual method, out of which 107 specimens were positive(3.5%) and the average detection time was 2.9 days(69.9 hours). In 1977, a total of 4,112 blood specimens were cultured by BACTEC 9240, out of which 170 specimens were positive(4.1%) and the average detection time was 18.2 hours. In BACTEC 9240, 79.4% of all cultured specimens was detected within 24 hours and 96.5% of all was detected within 48 hours. Coagulase negative Staphylococcus was the most frequently isolated organism, and followed by E. coli, Staphylococcus aureus, Bacillus, Enterobacter cloacae, and alpha-hemolytic Streptococcus.
CONCLUSIONS
BACTEC 9240 system showed the shortening of the detection time and the increase of positive rate in blood culture and we considered that the rapid blood culture report was more helpful to the clinicians.

MeSH Terms

Bacillus
Bacteremia
Coagulase
Diagnosis
Enterobacter cloacae
Humans
Staphylococcus
Staphylococcus aureus
Streptococcus
Coagulase