Ann Clin Microbiol.  2015 Dec;18(4):135-139. 10.5145/ACM.2015.18.4.135.

First Report of Yokenella regensburgei Isolated from the Wound Exudate after Disarticulation Due to Diabetic Foot Infection in Korea

Affiliations
  • 1Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. heejae.huh@samsung.com

Abstract

Yokenella regensburgei, a member of the family Enterobacteriaceae, is rarely isolated in humans. Here, we report a 71-year-old man with diabetic foot infection from which Y. regensburgei was isolated. Following debridement and disarticulation of the foot, an exudate specimen was obtained, from which Gramnegative bacilli were recovered. The organism was identified as Y. regensburgei using the Vitek 2 system (bioMerieux, USA) and 16S rRNA and gyrB gene sequencing. To our knowledge, this is the first case of Y. regensburgei isolation in Korea.

Keyword

Diabetic foot; DNA sequencing; Yokenella regensburgei

MeSH Terms

Aged
Debridement
Diabetic Foot*
Disarticulation*
Enterobacteriaceae
Exudates and Transudates*
Foot
Humans
Korea*
Sequence Analysis, DNA
Wounds and Injuries*

Figure

  • Fig. 1. (A) Gram stain microscopy of a colony grown on a blood agar plate. Gram-negative rods are shown (Gram stain, ×1,000). (B) Small, grayish, mucoid colonies on a blood agar plate, 2 days. (C) Small, colorless, mucoid colonies on a MacConkey agar plate, 2 days.


Reference

1.Kosako Y., Sakazaki R., Yoshizaki E. Yokenella regensburgei gen. nov., sp. nov.: a new genus and species in the family Enterobacteriaceae. Jpn J Med Sci Biol. 1984. 37:117–24.
2.Hickman-Brenner FW., Huntley-Carter GP., Fanning GR., Brenner DJ., Farmer JJ 3rd. Koserella trabulsii, a new genus and species of Enterobacteriaceae formerly known as Enteric Group 45. J Clin Microbiol. 1985. 21:39–42.
3.Abbott SL., Janda JM. Isolation of Yokenella regensburgei (" Koserella trabulsii") from a patient with transient bacteremia and from a patient with a septic knee. J Clin Microbiol. 1994. 32:2854–5.
4.Stock I., Sherwood KJ., Wiedemann B. Antimicrobial susceptibility patterns, beta-lactamases, and biochemical identification of Yokenella regensburgei strains. Diagn Microbiol Infect Dis. 2004. 48:5–15.
5.CLSI. Interpretive criteria for identification of bacteria and fungi by DNA target sequencing; approved guideline. CLSI document MM18-A. Wayne, PA: Clinical and Laboratory Standards Institute. 2008.
6.CLSI. Performance standards for antimicrobial susceptibility tes-ting; 25th informational supplement. CLSI document M100-S25. Wayne, PA: Clinical and Laboratory Standards Institute. 2015.
7.Kosako Y., Sakazaki R., Huntley-Carter GP., Farmer JJ. Yokenella regensburgei and Koserella trabulsii are subjective synonyms. Int J Syst Bacteriol. 1987. 37:127–9.
8.Stone ND., O'Hara CM., Williams PP., McGowan JE Jr., Tenover FC. Comparison of disk diffusion, VITEK 2, and broth micro-dilution antimicrobial susceptibility test results for unusual species of Enterobacteriaceae. J Clin Microbiol. 2007. 45:340–6.
9.Fajardo Olivares M., Blanco Palenciano J., Márquez Laffón I., Ruiz León JM. Yokenella regensburgei infection in a perimalleolar ulcer. Med Clin (Barc). 2005. 125:358–9.
10.Fill M., Stephens J. Abdominal abscess and septic shock secondary to Yokenella regensburgei. Internet J Infect Dis. 2009. 9:1–3.
11.Lo YC., Chuang YW., Lin YH. Yokenella regensburgei in an im-munocompromised host: a case report and review of the literature. Infection. 2011. 39:485–8.
12.Bhowmick T., Weinstein MP. A deceptive case of cellulitis caused by a Gram-negative pathogen. J Clin Microbiol. 2013. 51:1320–3.
Article
13.Jain S., Gaind R., Gupta KB., Dawar R., Kumar D., Paul P, et al. Yokenella regensburgei infection in India mimicking enteric fever. J Med Microbiol. 2013. 62:935–9.
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