Tuberc Respir Dis.  2004 Mar;56(3):289-296. 10.4046/trd.2004.56.3.289.

Metalloproteinase Plays a Role in Mucin Secretion

Affiliations
  • 1Department of Internal Medicine, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea. ymoh55@amc.seoul.kr
  • 2Asan Institute for Life Sciences, University of Ulsan, Seoul, Korea.

Abstract

BACKGROUND: Mucus hypersecretion in the patients with airway diseases represents poor prognosis as well as discomfort. However, there is no known therapy for its effective control. One important component of mucus is mucin, a glycosylated protein, which endows mucus with viscosity. We studied whether a proteinase has a role in mucin secretion and if so, which.
METHODS
(1) Inhibition of mucin secretion Group-specific proteinase inhibitors were tested to evaluate whether a proteinase belonging to a group of proteinases plays a role in mucin secretion. Phenylmethylsulfonyl fluoride(PMSF, a serine proteinase inhibitor), E-64(a cysteine proteinase inhibitor), Pepstatin(an aspartic proteinase inhibitor) and 1, 10-Phenanthroline(a metalloproteinase inhibitor) were treated into the Calu-3 cell line for 24 hours. The enzyme linked immunoabsorbant assay(ELISA) for MUC5AC was performed to evaluate the amount of mucin secretion and to compare with a control. (2) Stimulation of mucin secretion Matrix metalloproteinase-9(MMP-9), MMP-12 and TACE(TNF-alpha converting enzyme), which are known to be related with airway diseases, were used to be treated into Calu-3 for 24 hours. ELISA for MUC5AC was performed to evaluate the amount of mucin secretion and to compare with the controls.
RESULTS
(1) PMSF(10(-4)M), E-64(10(-4)M), Pepstatin(10(-6)M) and 1, 10-Phenanthroline(10(-4)M) reduced the MUC5AC secretion by 1 +/- 4.9%(mean +/- standard deviation; P=1.0 compared with the control), -6 +/- 3.9%(P=0.34), -13 +/- 9.7%(P=0.34) and 41 +/- 8.2%(P=0.03), respectively. (2) The amounts of MUC5AC secretion stimulated by MMP-9(250ng/ml), MMP-12(100ng/ml) and TACE(200ng/ml) were 103 +/- 6%(P=0.39), 102 +/- 8%(P=1.0) and 107 +/- 13%(P=0.39), respectively, compared with the controls.
CONCLUSION
Metalloproteinase(s) is (are) suggested to play a role in mucin secretion. It appears that metalloproteinases, other than MMP-9, MMP-12 or TACE, affect the mucin secretion in this in vitro model.

Keyword

Mucin; Secretion; Metalloproteinase

MeSH Terms

Cell Line
Cysteine Proteases
Enzyme-Linked Immunosorbent Assay
Humans
Metalloproteases
Mucins*
Mucus
Peptide Hydrolases
Prognosis
Serine Proteases
Viscosity
Cysteine Proteases
Metalloproteases
Mucins
Peptide Hydrolases
Serine Proteases
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