Hanyang Med Rev.  2015 Nov;35(4):215-221. 10.7599/hmr.2015.35.4.215.

The Current Status of Directed Differentiation Technology

• Lee SE
• Choi D

• KMID: 2129474
• DOI: http://doi.org/10.7599/hmr.2015.35.4.215

Abstract

The direct reprogramming of a terminally differentiated cell into another lineage using defined combinations of factors has fundamentally changed traditional concepts of the inalterability of differentiated cells. Many studies have achieved direct conversion into various cell types in recent years, and this strategy is considered to be a promising approach for inducing functional cells. Here, we review work on direct reprogramming, from the early pioneering studies to the most recent, including the discovery of novel reprogramming factors, molecular mechanisms, and strategies. We also discuss the applications of direct reprogramming and the perspectives and challenges of this novel technology.

Keyword

Cell Differentiation; Cellular Reprogramming; Transcription Factors; Regenerative Medicine

MeSH Terms

Cell Differentiation
Regenerative Medicine
Transcription Factors
Transcription Factors

Figure

• 

  Fig. 1 in vivo reprogramming of cardiac muscle by injecting various transcription factors. Direct reprogramming of cardiomyocytes in vivo is presently the most interesting application in regenerative therapy. A transfemoral catheter can be used to deliver Gata4/Mef2c/Tbx5 transcription factors to repair the injured myocardial wall after myocardial infarction. Lineage reprogramming can reduce the size of the cardiac infarct and enhance cardiac function. This strategy has advantages over induced iPSCs but several obstacles remain to be overcome.

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