J Korean Ophthalmol Soc.  2000 May;41(5):1073-1088.

The Effect of Nitric Oxide on Retinal Blood Flow Velocity Using Doppler Ultrasound aft erInt roducing into Vitreal Space in Pigmented Rabbit

• Rhee J
• Kim W
• Yang H

Abstract

The present study was undertaken to assess the effect of nitric oxide on the retinal vascular relaxation and retinal toxic effect.in pigmented rabbits. S-nitroso-N-acetyl-D, L-penicillamine[SNAP], as a NO onor, dissolved in dimethyl sulfoxide[DMSO]was injected into vitreal space.N-acetyl-D, L-penicillamine[NAP]was used as a control. We used Doppler Ultrasound to measures blood flow velocity.In the flow velocity between NAP group and SNAP group, the resistance index did not differ significantly for both central retinal artery and ciliary artery[p>0.05] 3 to 6 hours after NAP &SNAP injection. On electroretinographic study, a-wave amplitude of maximal combined response was reduced in SNAP treated group.In contrast, b/a ratio was increased between 3 hours and 6 hours after injection.Histologic examinations showed vacuolar degeneration and clumping and lysis of nuclei of photoreceptors.Lysosome and autophagic vacuoles were also noted in the cytoplasm of outer neuronal cells.In conjunction with above results, further studies about should be required to find the optimal concentration of NO & NO donor with minimal retinal toxicity and maximal therapeutic effect.

Keyword

Blood flow velocity; Doppler Ultrasound; Electroretinographic study; N-acetyl-D; L-penicillamine; Nitric oxide; Nitric oxide donor; Resistance index; Retinal toxic effect; S-nitroso-N-acetyl-D; L-penicillamine

MeSH Terms

Blood Flow Velocity*
Cytoplasm
Humans
Neurons
Nitric Oxide*
Rabbits
Relaxation
Retinal Artery
Retinaldehyde*
Tissue Donors
Ultrasonography*
Vacuoles
Nitric Oxide
Retinaldehyde