The Effect of Safflower Seed Extract on the Bone Formation of Calvarial Bone Model in Sprague Dawley rat

Kim, Sung Tae; Jhon, Gil Ja; Lim, So Hyoung; Cho, Kyoo Sung; Kim, Chong Kwan; Choi, Seong Ho

Abstract

The ultimate goal of periodontal therapy is the regeneration of periodontal tissue and repair of function. For more than a decade there have been many efforts to develop materials and methods of treatment to promote periodontal wound healing. Recently many efforts are concentrated on the regeneration potential of material used in oriental medicine. In some in vitro and in vivo experiments, there have been many evidences that these materials have an effect on bone regeneration. The purpose of this study was to evaluate histologically and radiologically in Sprague-Dawley rats the effects of safflower seed extracts on the regeneration of the calvarial defects surgically produced. So in this study, the critical size defects were surgically produced in the calvarial bone of 30 Sprague-Dawley rats using the 8mm trephine bur. The safflower seed extract was applied into the defect of each rat in experimental group, whereas nothing was applied into the defect of each rat in control group. Rats were sacrificed at 2, 4, 8 weeks following operation and histomorphometric and radiodensitometric analysis were performed. 1. The newly formed bone length was 102.91+/-22.05, 178.29+/-24.40 at 2 week in the each control, experimental group, 130.95+/-39.24, 242.62+/-50.33 at 4 week and 181.53+/-76.35, 240.36+/-22.00 at 8 week(unit, micrometer). In the 2, 4 week, there were statistically significant difference between control and experimental group(P<0.05). 2. The newly formed bone area was 2962.06+/-1284.48, 10648.35+/-1284.48 at 2 week, 5103.25+/-1375.88, 9706.78+/-1481.81 at 4 week, 8046.02+/-818.99, 12057.06+/- 740.47 at 8 week(unit, micrometer2). In every week, there were statistically significant difference between control and experimental group(P<0.05). 3. The radiopacity was 14.26+/-.33, 25.47+/-4.33 at 2 week, 20.06+/-9.07, 26.61+/-2.78 at 4 week, 22.99+/-3.76, 27.29+/-1.54 at 8 week(unit, %). In the 2 week, there was statistically significant difference between control and experimental group(P<0.05). In conclusion, the results of the present study suggest that safflower seed extract initially has an effect on the newly formed bone area, length and radiopacity when it is applied to the calvarial defect of Sprague - Dawley rat. Then. the material has an effect on newly formed bone area and length.