Lab Anim Res.  2010 Dec;26(4):339-343. 10.5625/lar.2010.26.4.339.

Modifying Effect of Indole-3-carbinol on the Rat Colon Carcinogenesis

• Jo H
• Han BS
• Kang JS
• Jang DD
• Kim K
• Ahn B
• Kim DJ

• KMID: 2114701
• DOI: http://doi.org/10.5625/lar.2010.26.4.339

Abstract

Indole-3-carbinol (I3C) found in various cruciferous vegetables has been shown to exert anti-carcinogenic activity in several target organs. Our study was conducted to assess the modifying effect of I3C on the development of colon tumor induced by azoxymethane (AOM). Eighty-seven male F344 rats were divided into 5 groups and were treated with AOM followed by I3C 100 or 300 ppm, AOM alone, I3C alone, and non-treatment, respectively. The animals were subcutaneously injected with AOM. Then diet containing I3C were fed to the rats for 37 weeks. All rats were sacrificed at 40 weeks. Liver and kidney weights of rats treated with I3C at doses of 100 or 300 ppm were significantly increased compared to those of the control group. Colonic tumor incidence and multiplicity of rats treated with I3C at doses of 100 and 300 ppm were not significant compared to those of AOM alone group. In the pathological examination, most of tumors were classified with adenoma and adenocarcinoma in the small and large intestine. These results demonstrated that I3C may have not chemopreventive effect on the rat colon carcinogenesis.

Keyword

Indole-3-carbinol (I3C); colon carcinogenesis; azoxymethane (AOM); rat

MeSH Terms

Adenocarcinoma
Adenoma
Animals
Azoxymethane
Colon
Diet
Humans
Incidence
Indoles
Intestine, Large
Kidney
Liver
Male
Rats
Rats, Inbred F344
Vegetables
Weights and Measures
Azoxymethane
Indoles

Figure

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  Figure 1. Experimental design.

Reference

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