Tuberc Respir Dis.  2010 Oct;69(4):256-264. 10.4046/trd.2010.69.4.256.

Moxifloxacin Alleviates Oleic Acid-provoked Neutrophilic Respiratory Burst in the Rat Lung through the Inhibition of Cytosolic Phospholipase A2

Affiliations
  • 1Department of Physiology, Daegu Catholic University School of Medicine, Daegu, Korea. leeym@cu.ac.kr

Abstract

BACKGROUND
According to the notion of the immunoregulatory functions of moxifloxacin (MFX), the effect of MFX on the neutrophilic respiratory burst in conjunction with the expression of cytosolic phospholipase A2 (cPLA2) was investigated.
METHODS
The effects and possible mechanisms of MFX on neutrophilic respiratory burst in oleic acid (OA)-induced acutely injured rats lung and OA-stimulated, isolated murine neutrophils were probed, associated with the expression of cytosolic phospholipase A2 in vivo and in vitro.
RESULTS
In the OA-induced acutely-injured lungs, neutrophils were accumulated, which was attenuated by MFX. The parameters denoting a neutrophilic respiratory burst, such as nitro blue tetrazolium reaction, cytochrome-c reduction, neutrophil aggregation, H2O2 production in neutrophils revealed increased neutrophilic respiratory burst by OA, and MFX decreased all of these parameters. In addition, the enhanced expression of cPLA2 in the lung and isolated murine neutrophils by OA were decreased by MFX.
CONCLUSION
MFX suppresses the OA-induced neutrophilic respiratory burst by the suppression of cPLA2 in neutrophils.

Keyword

Oleic Acid; Neutrophils; Phospholipases A2, Cytosolic; Moxifloxacin
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