J Korean Ophthalmol Soc.  2005 Sep;46(9):1575-1581.

Axoplasmic Transport of Herpes Simplex Virus Co-Cultured with Ciliary Nerve

• Kang SW
• Ko MK

Abstract

PURPOSE
To investigate the replication of HSV within cultured cell and axonal transport of HSV within the axon of the ciliary nerve following the injection of HSV into a cultured ciliary nerve. METHODS: The explant of the ciliary nerve was cultured with a medium containing nerve growth factor for 30 days when the suspension of HSV-1 (Kos strain) was introduced into the culture dish to co-culture with the ciliary nerve. The ciliary nerve was examined with transmission electron microscopy 30 days after culture and 6 days after co-culture with HSV. RESULTS: The ultrastructure of the explant of the ciliary nerve co-cultured with HSV showed that the viral capsid acquired a viral envelope and viral core, and a capsid and inclusion body within the nucleus. The enveloped virus was scattered within the vesicles of the cytoplasm. The virus-like particles were identified at the axonal fibers. CONCLUSIONS: The co-culture of the explant of the ciliary nerve and HSV showed the replicative process of the HSV within the cultured cell. The virus-like particles within the axon showed the evidence axonal transport of the virus under culture conditions.

Keyword

Axonal transport; Ciliary nerve; Culture; Herpes simplex virus; Ultrastructure

MeSH Terms

Axonal Transport*
Axons
Capsid
Cells, Cultured
Coculture Techniques
Cytoplasm
Herpes Simplex*
Herpesvirus 1, Human
Inclusion Bodies
Microscopy, Electron, Transmission
Nerve Growth Factor
Simplexvirus*
Nerve Growth Factor