J Korean Ophthalmol Soc.
2001 Sep;42(9):1331-1335.
Proliferation of Corneal Endothelial Cells by Delivery of SV40 Large T Antigen
- Affiliations
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- 1Department of Ophthalmology and Visual Science, Kangnam St. Mary's Hospital.
- 2Catholic University Medical College, Research Institutes of Medical Science, Seoul, Korea.
- 3Department of Ophthalmology and Visual Science, Washington University School of Medicine.
Abstract
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PURPOSE: To determine whether the delivery of the SV40 large T-antigen is a feasible method for transiently inducing proliferation of corneal endothelial cells, we delivered liposome-protein complex into bovine corneal endothelial cells(BCEC).
METHOD: SV40 large T-antigen protein was introduced into BCEC and positive cells were identified by immunohistochemistry. Quiescent BCECs were double-labeled using BrdU as a measure of de novo DNA synthesis and the Ki-67 was detected by standard immunohistochemical methods.
RESULT: The treatment of quiescent BCECs with large T antigen caused an increase in BrdU incorporation and Ki-67 expression. It was tested by time-course study.
CONCLUSION
This finding suggests that liposome-mediated delivery of transforming proteins could be a method to transiently induce corneal endothelial cell proliferation.
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