J Korean Soc Emerg Med.  2003 Mar;14(1):29-37.

Immune Alteration in Rebleeding

• Lee HS
• Cho KH
• Sohn DK
• Park JS
• Lee JH
• Chung SP

Abstract

PURPOSE
This study evaluates whether the adaptation mechanism could modulate immunosuppression following a hemorrhage.
METHODS
Minor hemorrhage (10% of total blood volume) was induced in the rat model, 1, 2, 4, and 7 days before the main hemorrhage. The immune responses were observed by measuring Con A (Concanavalin A) stimulated proliferative capacity of the peripheral lymphocyte subpopulations, and the Interleukin-2 (IL-2) release from splenocytes.
RESULTS
The proliferative capacity of the splenocytes (SPC) decreased in two days interval model with hemorrhages more than 20% of the total blood volume. The SPC increased in the group with 10% hemorrhage pretreatment 7 days prior to 20% main hemorrhage than it was for 20% or 30% main hemorrhage only with cardiac puncture groups. The SPC increased on the first day than it was on the fourth day after the 20% main hemorrhage, in the pretreatment group with 7 days interval. The amount of IL-2 release by the splenocytes was higher in the 10~20% group (10% pretreatment hemorrhage and 20% main hemorrhage) than it was in the 0~30% group, when the hemorrhage interval was 7 days, and it was higher on the first day than on the fourth day after the second hemorrhage in the 10~20% group.
CONCLUSION
The immune response varied depending on the hemorrhage interval following pretreatment, and it increased after the main hemorrhage that, by itself, would cause immunosuppression. But this effect, however, was only observed during a short period (about 1 day) following the second hemorrhage.

Keyword

Hemorrhage; Immunosuppression; Tolerance

MeSH Terms

Blood Volume
Hemorrhage
Immunosuppression
Interleukin-2
Lymphocyte Subsets
Models, Animal
Punctures
Interleukin-2