Korean J Gastroenterol.  2001 Dec;38(6):411-418.

Alimentary Tract: Detection of Multiple Strains of Helicobacter pylori Infection in Koreans

• Park SH
• Choi MG
• Kim JK
• Chung IS
• Sun HS
• Park DH
• Kim BW
• Lee HS
• Choi H
• Choi KY
• Lee IS
• Han SW

Abstract

BACKGROUND/AIMS: The aim of this study was to determine intra-variation of Helicobacter pylori (H. pylori) strains of each patient and to investigate the affection of multiple strains of H. pylori infection to the disease outcome in Korea.
METHODS
Thirty patients who were H. pylori-positive and had gastritis (n=10), duodenal ulcer (n=10) and gastric adenocarcinoma (n=10) were included in this study. Their disease were confirmed endoscopically and histologically. Their H. pylori were cultured. Five H. pylori colonies were picked randomly from the primary cultures. Using primer 1254, random amplified polymorphic DNA fingerprinting (RAPD) analysis was carried out. Restriction fragment length polymorphism (RFLP) patterns for the subtypes of ureC, cagA, and vacA were also investigated.
RESULTS
RAPD method confirmed that four of gastritis patients (40%), four of duodenal ulcer patients (40%), and five of gastric adenocarcinoma patients (50%) were infected with multiple strains of H. pylori. The intra-variation of RFLP patterns for the subtypes of ureC, cagA, and vacA were detected in the three groups.
CONCLUSIONS
Multiple strains of H. pylori and the subtypes of cagA and vacA may not affect the outcome of the gastrointestinal disease.

MeSH Terms

Adenocarcinoma
DNA Fingerprinting
Duodenal Ulcer
Gastritis
Gastrointestinal Diseases
Helicobacter pylori*
Helicobacter*
Humans
Korea
Polymorphism, Restriction Fragment Length