Korean J Gynecol Oncol Colposc.  2004 Sep;15(3):231-239.

Establishment of General Primers and Oligo-DNA Chip for the Detection and Typing of Human Papillomavirus

• Kwon JE
• Kim YS
• Kim SJ
• Na YJ
• Kim IH
• Lee SY
• Ko JJ
• Lee SH

Abstract

OBJECTIVE
To establish the use of a HPV oligo-DNA chip for early diagnosis and prognosis assessment in cervical cancer.
METHODS
HPV PCR general primers and a oligo-DNA chip have been constructed by using HPV genomic sequences on the webs and computer programs. With a total of 344 random cervical scrapes, their efficacies were explored based on pathology.
RESULTS
All subjects (n=48) with diagnosis of low squamous intraepithelial lesion (LSIL) were positives for both HPV PCRs and oligo-DNA chip tests, whereas 44 out of 286 subjects (264 cases of NIL and 22 cases of ASCUS) were detected HPV positives. The proportion of each genotypes in HPV positive subjects were 38.0% (HPV-16), 16.3% (HPV-58), 9.8% (HPV-52) and 35.9% (others).
CONCLUSION
This study gives favorable result to establish a novel method using HPV oligo-DNA chip with PCR general primers, based on the clinical trials in random subjects although the scale is small in size. In the future, a diverse of comparative and systematic clinical studies should be followed.

Keyword

Human papillomavirus; General primer; HPV oligo-DNA chip; Cervical cancer

MeSH Terms

Diagnosis
Early Diagnosis
Genotype
Humans*
Pathology
Polymerase Chain Reaction
Prognosis
Uterine Cervical Neoplasms