Korean J Nephrol.  2005 Jan;24(1):57-63.

The Usefulness of Measuring Urinary Proteins by SDS-PAGE in Children with Type I DM without Overt Proteinuria

• Cho MH
• Kim BM
• Jang HS
• Ko CW
• Koo JH
• Song KE
• Chung KY

Abstract

BACKGROUND
The present study was done to evaluate the usefulness of SDS-PAGE in measuring glomerular proteins and tubular proteins in patients with type I DM without overt proteinuria. METHODS: The study population consisted of 76 children with type I DM who have been participated in the Taegu Diabetic Camp from 1997 to 2000. We measured urine albumin, NAG, beta2-microglobulin, creatinine level in urine samples collected for 12 hours in 22 children and simultaneously we analyzed urinary proteins by SDS-PAGE. In remainder 54 children, we measured urine albumin, NAG, creatinine level in random morning urines and urinary proteins by SDS-PAGE. RESULTS: In 22 of 76 children, urinary albumin-to-creatinine ratio (mg/mg), NAG (U/g) and beta2-microglobulin (microgram/g) were 0.021, 2.99 and 170.2, respectively. Positive correlation between urine albumin and NAG was statistically significant (r=0.51, p< 0.05). No significant relations between urinary proteins, such as albumin, NAG, beta2-microglobulin, and clinical characteristics, such as age, duration of illness, HbA1c, were observed. In diagnosing `microalbuminuria', sensitivity of SDS-PAGE was 63% and band of tubular proteins was not detected in any children. CONCLUSION: We thought that SDS-PAGE could be one of useful methods in diagnosing early stage of diabetic nephropathy. The more large scale study is necessary.

Keyword

SDS-PAGE; Type I diabetes mellitus; Diabetic nephropathy; lomerular and tubular protein

MeSH Terms

Child*
Creatinine
Daegu
Diabetic Nephropathies
Electrophoresis, Polyacrylamide Gel*
Humans
Proteinuria*
Creatinine