Korean J Pediatr Hematol Oncol.  1997 Apr;4(1):48-54.

A Role of gamma-subunit in FcgammaRII Signal Transduction of Platelets

Abstract

BACKGROUND: The FcgammaRII receptor, the only kind of Fc receptor in the platelet, plays important role for immune-complex mediated platelet activation, which occurs in Heparin induced thrombocytopenia(HIT) or antiphospholipid syndrome. The purpose of this research is to find out the role of gamma-subunit in FcgammaRII signal transduction of platelets.
METHODS
After cross-linking the FcgammaRII receptors with IV.3 anti FcgammaRII specific antibody and Rabbit anti mouse antibody, we made the platelet lysate in the RIPA EDTA lysis buffer condition, and performed anti gamma-subunit immunoprecipitation. After polyacylamide gel electrophoresis and Western transfer, we probed the nitrocellulose membrane of gamma-subunit immunoprecipitate with anti gamma-subunit antibody.
RESULTS
The mobility shift of gamma-subunit was observed upon FcgammaRII cross-linking.
CONCLUSION
The gamma-subunit is activated upon FcgammaRII cross-linking, which means the gamma-subunit is involved in FcgammaRII signal transduction of platelet.

Keyword

Platelet; FcgammaRII receptor; gamma-subunit

MeSH Terms

Animals
Antiphospholipid Syndrome
Blood Platelets
Collodion
Edetic Acid
Electrophoresis
Heparin
Immunoprecipitation
Membranes
Mice
Platelet Activation
Receptors, Fc
Signal Transduction*
Collodion
Edetic Acid
Heparin
Receptors, Fc
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