Korean J Physiol Pharmacol.  2001 Aug;5(4):307-313.

Decreased expression of aquaporin-2 water channels in the kidney in rats treated with reserpine

• Lee JU
• Oh YW
• Kim S

Abstract

Whether there exists a Sympathetic neural mechanism regulating the expression of aquaporin (AQP) water channels in the kidney was investigated. Male Sprague-Dawley rats were treated with reserpine (1 mg/kg, IP), and the expression of AQP1-4 proteins was determined in the kidney one day thereafter. Following the treatment with reserpine, the systolic blood pressure measured in a conscious state was significantly decreased in the experimental group compared with that in the control (83+/-8 vs 124+/-6 mmHg n=6 each, P<0.05). The expression of AQP2 proteins was decreased in the cortex, outer medulla, and inner medulla. The decrease of AQP2 proteins was in parallel in the membrane and the cytoplasmic fractions, suggesting a preserved AQP2 targeting. No significant changes were observed in the expression of AQP1, AQP3, or AQP4. Neither basal nor AVP-stimulated formation of cAMP was significantly altered. These results suggest that the sympathetic nervous system has a tonic stimulatory effect specifically on the expression of AQP2 water channels in the kidney.

MeSH Terms

Animals
Aquaporin 2*
Aquaporins*
Blood Pressure
Cytoplasm
Humans
Kidney*
Male
Membranes
Rats*
Rats, Sprague-Dawley
Reserpine*
Sympathetic Nervous System
Aquaporin 2
Aquaporins
Reserpine